Ceftiofur is a broad-spectrum antibiotic of the cephalosporin group, effective against Gram-positive, gram-negative including lactamase producing strains.
The Ceftiofur ELISA Test Kit produced by Meizheng is fast, simple, accurate and high sensitivity, with operation time 45min, which can minimize the operating error and working intensity
Order No.: ME69AER
LOD: 1 ppb
Ceftiofur ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Ceftiofur antigen is precoated on the wells. The precoated antigen compete the Ceftiofur antibody (antibody solution) with Ceftiofur in the sample, Ceftiofur antibody binds to the Ceftiofur enzyme conjugate. After the color development of TMB substrate, the absorbance value of the sample is negatively correlated with the Ceftiofur contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Ceftiofur in the sample can be calculated.
Materials Required But Not Provided
—- Microplate Reader (450nm/630nm)
—- Balance (sensibility: 0.01g)
—- Centrifuge tube: 5mL/7mL, 50mL
—- Micropipette: Single channel pipettte (10µL-100µL, 100µL-1000µL)
Multi-channel pipette (30µL-300µL)
—- Deionized water
—- 18M concentrated sulfuric acid (H2SO4)
[Provided Materials and Reagent]
|Antigen coated plate||96T|
|5 vials of standard||1mL each|
|Concentrated sample diluting buffer||40mL|
|Concentrated Wash Buffer (10×)||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 1ppb, 3ppb, 9ppb, 27ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.