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    Animal Tissue Antibiotic Ceftiofur ELISA Test Kit

    Qualitative or quantitative analysis of Ceftiofur in animal tissue (chicken, pork).

    Summary

    Ceftiofur is a broad-spectrum antibiotic of the cephalosporin group, effective against Gram-positive, gram-negative including lactamase producing strains.

    The Ceftiofur ELISA Test Kit produced by Meizheng is fast, simple, accurate and high sensitivity, with operation time 45min, which can minimize the operating error and working intensity

    Order No.: ME69AER

    LOD: 1 ppb

    Package: 96T

    Use Principles

    Ceftiofur ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Ceftiofur antigen is precoated on the wells. The precoated antigen compete the Ceftiofur antibody (antibody solution) with Ceftiofur in the sample, Ceftiofur antibody binds to the Ceftiofur enzyme conjugate. After the color development of TMB substrate, the absorbance value of the sample is negatively correlated with the Ceftiofur contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Ceftiofur in the sample can be calculated.

    Cross-reactivity

    Ceftiofur…………………………………………………..……100%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm)

    ---- Homogenizer

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Centrifuge tube: 5mL/7mL, 50mL

    ---- Micropipette: Single channel pipettte (10µL-100µL, 100µL-1000µL)

    Multi-channel pipette (30µL-300µL)

    1. Reagent:

    ---- Acetonitrile

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    [Provided Materials and Reagent]

    Component96 wells
    Antigen coated plate96T
    5 vials of standard1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated sample diluting buffer40mL
    Concentrated Wash Buffer (10×)40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 1ppb, 3ppb, 9ppb, 27ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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