Principle of Test
Tylosin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Tylosin antigen is precoated on the wells. The precoated antigen compete the Tylosin antibody (antibody solution) with Tylosin in the sample, anti-Tylosin antibody binds to the Tylosin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Tylosin concentration of the samples is derived.
Scope of Application
Qualitative or quantitative analysis of carbendazim in honey.
| Manufacturer | Meizheng |
| Order No. | SC0295 |
| Analyte | Tylosin |
| Sample Type | Honey |
| Methodology | Indirect competitive enzyme-labeled immunoassay |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Tylosin………………………………………100% Tilmicosin………………………..…………105% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.5 ppb |
| Limit of detection
| 20ppb |
| Percent Recovery, % | 100±30% |
| Precision (CV %):
| Intra-lab assay: CV%≤10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 96T |
| 6 vials of standard | 1mL each |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Concentrated Sample Diluting Buffer | 40mL |
| Package Insert | 1 |
| Plate cover sheet | 2 |
| Testing Report | 1 |
Note: The concentrations of the standards are 0ppb, 0.5ppb,1.5ppb, 4.5ppb, 13.5ppb and 40.5pp.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Vortex mixer
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Graduated pipette: 10mL
—- Centrifuge tube: 5mL/7mL, 50mL
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30-300µL)
- Reagent:
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
