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Corn and Feed Mycotoxin Fumonisins ELISA Test Kit

The Fumonisins(FB) ELISA Kit is an indirect competitive ELISA kit for the quantitative analysis of fumonisins in feed, corn, and corn by- products.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Fumonisin is one of the mycotoxins produced by F. Moniliforme. And has 28 derivatives. FB is the most universal one of the derivatives, which mainly contaminate corn and their products. FB has a toxin effects to animals because of its powerful toxicity. As study shown that FB can cause the Leukoencephalopathy softening syndrome of horses and Pulmonary edema syndrome of pigs and can also induce human esophageal cancer, liver cancer, gastric cancer, and other diseases, so it poses a threat to husbandry and human health.

This kit is toxin detection products from the latest ELISA technology research and development, it has advantages of rapid, simple, accurate and high sensitivity compared with the instrument analysis technology, and its operation time only 15 min and can also minimize the work intensity and operation error.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM1296
AnalyteFumonisins
Sample TypeFeed,Corn, and Corn by- products.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityFB…………………………………………100%
FB2…………………………………………71%
FB3…………………………………………85%
 Calculate ResultAnalyze the result by using the software
Sensitivity5 ppb
Limit of detection250 ppb
Percent Recovery, %100±20%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent:

ComponentSpecs
Antigen coated plate96 wells
5 vials of standard *1mL each
FB Enzyme conjugate12mL
FB Antibody Solution12mL
Substrate10mL×2
Concentrated Wash Buffer (10×)40mL
Package Insert1
Test report1
Plate cover2

 Note: The concentration of the standards is 0µg/kg, 5µg/kg, 15µg/kg, 45µg/kg, 135µg/kg.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate reader: 450nm/630nm

—- Vortex

—- Centrifuge (or funnel)

—- Balance: sensibility: 0.01g

—- Graduate Pipette: 10mL

—- Aurilave

—- Graduate cylinder: 50mL

—- Conical flask with stopper: 100mL

—- Centrifuge tube: 7mL

—- Quantitative analysis filter paper

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)

  1. Reagent:

—- Methanol (AR)

—- Deionized water

—- 18M concentrated sulfuric acid (H2SO4)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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