Background:
Folic acid is one of the B vitamins. Folic acid is the form of folate used to treat anemia caused by folic acid deficiency. Folic acid is also used as a supplement by women during pregnancy to prevent neural tube defects in the baby.
HPLC method is now widely used in the detection of folic acid, but its high cost and complex operation make it unacceptable.
The Folic Acid (FA) ELISA Test Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.
Use Principles:
Folic acid (FA) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The folic acid antigen is precoated on the wells. The precoated antigen compete the folic acid antibody (antibody solution) with folic acid in the sample, folic acid antibody binds to the folic acid enzyme conjugate. After the color development of TMB substrate, the absorbance value of the sample is negatively correlated with the folic acid contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of folic acid in the sample can be calculated.
| Manufacturer | Meizheng |
| Order No. | SC0114 |
| Analyte | Folic acid |
| Sample Type | Milk powder, rice flour, raw milk, etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Folic acid………………………………………100% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 1ppb |
| Limit of detection | Milk powder………………………………100ppb Rice flour…………………………………150ppb Raw milk, cow milk………………………10ppb |
| Percent Recovery, % | 100±20% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 96T |
| 5 vials of standard | 2mL each |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated sample diluting buffer (10×) | 40mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Package Insert | 1 |
| Testing Report | 1 |
| Plate Cover Sheet | 2 |
| Component | 96 wells |
Note: The concentrations of the standards are 0ppb, 1ppb, 3ppb, 9ppb and 27ppb.
Materials Required But Not Provided
- Devices:
---- Microplate Reader (450nm/630nm)
---- Oscillator
---- Vortex
---- Centrifuge
---- Balance (sensibility: 0.01g)
---- Transfer pipette: 10mL
---- Rubber suction bulb
---- Centrifuge tube: 50mL
---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)
Multi-channel pipette (30µL-300µL)
- Reagent:
---- Methanol (AR)
---- Deionized water
--- 18M concentrated sulfuric acid (H2SO4)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
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