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    Folic Acid ELISA Test Kit for Milk Powder, Rice flour and Dairy

    Qualitative or quantitative analysis of folic acid in milk powder, rice flour, raw milk, etc.

    Brochure-Dairy Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Folic acid is one of the B vitamins. Folic acid is the form of folate used to treat anemia caused by folic acid deficiency. Folic acid is also used as a supplement by women during pregnancy to prevent neural tube defects in the baby.

    HPLC method is now widely used in the detection of folic acid, but its high cost and complex operation make it unacceptable.

    The Folic Acid (FA) ELISA Test Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.

     Use Principles: 

    Folic acid (FA) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The folic acid antigen is precoated on the wells. The precoated antigen compete the folic acid antibody (antibody solution) with folic acid in the sample, folic acid antibody binds to the folic acid enzyme conjugate. After the color development of TMB substrate, the absorbance value of the sample is negatively correlated with the folic acid contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of folic acid in the sample can be calculated.

    ManufacturerMeizheng
    Order No.SC0114
    AnalyteFolic acid
    Sample TypeMilk powder, rice flour, raw milk, etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityFolic acid………………………………………100%
     Calculate ResultAnalyze the result by using the software
    Sensitivity1ppb
    Limit of detectionMilk powder………………………………100ppb
    Rice flour…………………………………150ppb
    Raw milk, cow milk………………………10ppb
    Percent Recovery, %100±20%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96T
    5 vials of standard2mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated sample diluting buffer (10×)40mL
    Concentrated Wash Buffer (10×)40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2
    Component96 wells

     Note: The concentrations of the standards are 0ppb, 1ppb, 3ppb, 9ppb and 27ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm)

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Rubber suction bulb

    ---- Centrifuge tube: 50mL

    ---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

    Multi-channel pipette (30µL-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    --- 18M concentrated sulfuric acid (H2SO4)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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