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    Grains and Feed Ochratoxin A (OTA) ELISA Test Kit

    The Ochratoxin A (OTA) ELISA Kit is an indirect competitive ELISA for the quantitative analysis of Ochratoxin A in corn, wheat, feed, etc.

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Ochratoxin A (OTA) is a toxic metabolite produced by Aspergillus flavus and Penicillium genera. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenic, mutagenic and carcinogenic.

    OTA was considered to be the second important and harmful mycotoxin in the family following with Aflatoxin flavus. As the OTA producing fungi exist widely in nature, especially higher yield in cereals and cereals products, coffee, cocoa contaminated with OTA, it has been potential threat for human health and development of animal husbandry.

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM1096-1
    AnalyteOchratoxin A
    Sample TypeCorn, Wheat,Feed, etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityOchratoxin A………………………………100%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.05 ppb
    Limit of detectionCorn, wheat, flour, etc. ………………1.25μg/kg
    Feed………………………………….…2.5μg/kg
    Percent Recovery, %100±20%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent:

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1mL each
    OTA HRP-conjugate12mL
    OTA Antibody Solution6mL
    Concentrated sample diluting solution (20X)40mL
    Substrate A6mL
    Substrate B6mL
    Stop Solution6mL
    Concentrated Wash Buffer (10X)40mL
    Package Insert1
    Testing Report1
    Plate cover2

     Note: Please dilute the concentrated standards before use.

    The concentration of the standards is 0μg/kg, 0.05μg/kg, 0.15μg/kg, 0.45μg/kg, 1.35μg/kg, 4.05μg/kg.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex (or oscillator)

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Aurilave

    ---- Centrifuge tube: 50mL

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30µL-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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