Background:
Sulfonamides (SAs) are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.
The Sulfonamides (SAs) ELISA Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.
| Manufacturer | Meizheng |
| Order No. | SC0083 |
| Analyte | Sulfonamides |
| Sample Type | Honey |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Sulfamethazine 100% Sulfadiazine 797% Sulfamonomethoxine 1360% Sulfaquinoxaline 120% Sulfachloropyridazine 170% Sulfachloropyrazine sodium 2113% Sulfadimoxine 1054% Sulfamerazine 335% Sulfadoxine 1057% Sulfadimethoxine 849% Phthalylsulfathiazole 90% Sulfapyridine 62% Sulfapyridine 55% Sulfamethizole 518% Sulfamidin 49% Benzenesulfonamide 21% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 2 ppb |
| Limit of detection | Honey 2ppb |
| Percent Recovery, % | 110±20% |
| Precision (CV %): | Intra-lab assay: CV<10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 5 vials of standard | 1mL each |
| Concentrated standard 1ppm | 1mL |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Auxiliary Extracting Agent | 10mL |
| Concentrated sample diluting buffer | 40mL |
| IFU | 1 |
| Testing Report | 1 |
| Plate cover sheet | 2 |
Note: The concentrations of the standards are 0ppb, 2ppb, 6ppb, 18ppb and 54ppb.
Materials Required But Not Provided
- Devices:
---- Microwell plate Reader (450nm/630nm)
---- Vortex
---- Balance (sensibility: 0.01g)
---- Nitrogen evaporator
---- Centrifuge
---- Transfer pipette: 10mL
---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)
Multi-channel pipette (30µL-300µL)
- Reagent:
---- Deionized water
---- Concentrated hydrochloric acid (AR)
---- NaOH (AR)
----Disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O) (AR)
---- Sodium dihydrogen phosphate (NaH₂PO₄·H₂O) (AR)
---- Ethyl acetate (AR)
---- N-hexane (AR)
---- Concentrated sulfuric acid (18mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
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