Principle of the Test
This immunoassay test uses a double antibody sandwich format. An antibody specific for Listeria is sprayed in a line on the surface of a membrane comprising a “test line”. A second antibody reagent, also recognizing Listeria and labeled with colloidal gold, is contained within a reagent pad upstream from the test line. As the sample moves from the filter pad, the antibody gold reagent specifically binds Listeria and moves with the liquid sample into the test membrane. The sample passes through the test line where the immobilized second Listeria antibody captures the protein–antibody–gold complex, causing the formation of an antibody–protein “sandwich” and development of red color at the test line. Antibody–protein sandwiches are not formed in the absence of the Listeria, resulting in no red color development at the test line.
Sensitivity
3×105 ~3×106 CFU/mL
Contents of Kit
No. | Description | Quantity |
1 | Listeria Test Cassette | 50 |
2 | Disposable dropper | 50 |
3 | Desiccant | 50 |
4 | Package Insert | 1 |
Storage and Shelf Life
- Storage: Store kit at room temperature (4-30℃). DO NOT FREEZE!
- Shelf life: 12 months.
- Production date, expiration date and lot number please refer to the label on the out package.
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