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    Milk Powder Bovine Lactoferrin ELISA Test Kit

    Order No.: SC0286

    Lactoferrin (LF) is an important non-heme iron-binding glycoprotein in milk and a monomeric glycoprotein with bactericidal activity in neutrophil granules. Lactoferrin not only participates in the transport of iron, but also has powerful biological functions such as broad-spectrum antibacterial, antioxidant, anticancer, and immune system regulation.

    【Principles】

    Bovine Lactoferrin ELISA Test Kit is a double-antibody sandwich enzyme-linked immunoassay. Pre-coated bovine lactoferrin antibody on the wells, the pre-coated bovine lactoferrin antibody and HRP enzyme conjugate antibody are combined with the bovine lactoferrin in the sample at the same time to form an antibody-bovine lactoferrin-HRP-enzyme-conjugate complex, which is colored by TMB substrate. The absorbance value of the sample is positively correlated with the content of bovine lactoferrin. Compared with the standard curve and multiplied by the corresponding dilution factor, the content of bovine lactoferrin in the sample can be calculated.

    【Scope of Application】

    Qualitative or quantitative analysis of bovine lactoferrin content in milk powder.

    Cross-reactivity

    Bovine lactoferrin…………………………………….………100%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Constant Temperature Incubator

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Rubber suction bulb

    ---- Centrifuge tube: 2mL, 7mL, 50mL

    ---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

    Multi-channel pipette (30-300μL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component 96 wells
    Antibody coated plate12 × 8 wells
    5 vials of standard1mL each
    Enzyme Conjugate6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Extraction Buffer50mL
    Package Insert1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0μg/kg, 20μg/kg, 60μg/kg, 180μg/kg, and 540μg/kg.

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