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Pesticides Sodium Pentachlorophenol ELISA Test Kit for Meat

【Summary】

Sodium Pentachlorophenol is the sodium salt of pentachlorophenol, which belongs to organochlorine pesticides and can be used as insecticides, antibacterial agents, and preservatives. Sodium pentachlorophenate is easy to accumulate in animals and can enter the human body through the food chain, seriously endangering human health.

Use Principles

Sodium Pentachlorophenol ELISA Kit is an indirect competitive

enzyme-labeled immunoassay. The Sodium Pentachlorophenol antigen is precoated on the wells. The precoated antigen compete the Sodium Pentachlorophenol antibody (Antibody Solution) with Sodium Pentachlorophenol in the sample, Sodium Pentachlorophenol antibody binds to the Sodium Pentachlorophenol Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Sodium Pentachlorophenol contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Sodium Pentachlorophenol in the sample can be calculated.

Scope of Application

Qualitative or quantitative analysis of Sodium Pentachlorophenol concentrations in samples of chicken, and duck.

【Cross Reactivity】

Sodium Pentachlorophenol 100%

【Materials Required But Not Provided】

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Termovap Sample Concentrator

—- Oscillator

—- Centrifuge

—- Constant Temperature Incubator

—- Balance (sensibility: 0.01g)

—- Graduated pipette: 10mL

—- Rubber Bulb Syringe

—- Centrifuge tube: 10mL,50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL).  Multi-channel pipette (30-300µL)

  1. Reagent:

—- Deionized water

—- Sodium Hydroxide (NaOH) (AR)

—- Ethyl Acetate (AR)

—- 18M Concentrated Sulfuric Acid (H2SO4)

【Provided Materials and Reagent】

Component96 wells
Antigen coated plate96T
5 vials of Standard2mL each
High Concentration Standard (1ppm)2mL
Enzyme Conjugate12mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer (8×)40mL
Package Insert1
Testing Report1
Plate cover sheet2

Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, and 5.4ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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