Search

In Stock

Sulfonamides ELISA Test Kit for Poultry Tissues, Aquatic Tissue and Serum

Scope of Application

The Sulfonamides ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of sulfonamides in samples of poultry tissues, aquatic tissue, serum, etc.

Order No: ME08BER

Summary

Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human.

They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their

effectiveness against gram-negative and gram-positive organisms.

The detection method is sensitive, fast, simple, and specific, only need 45mins for operating and a small amount of sampling.

Use Principles

The Sulfonamides ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen

compete the antibody (antibody solution) with sulfonamides in the sample, anti-sulfonamides antibody binds to the HRP enzyme conjugate. Then

pipette the substrate solution to the wells to convert the color. The color of unknown samples is compared to the color of the standards and the

sulfonamides concentration of the samples is derived.

SENSITIVITY (DETECTION LIMIT)

—- Sensitivity: 0.5ppb

Limit of detection

Tissue sample ……………………………………………………………………………….. 4ppb

Serum sample……………………………………………………………………………….. 5ppb

Cross-reactivity

Sulfadiazine …………………………………………………………………………………..100%

Sulfadoxine ……………………………………………………………………………………181%

Sulfamonomethoxine……………………………………………………………………..150%

Phthalylsulfathiazole (PST)……………………………………………………………….90%

Sulfamethoxydiazine………………………………………………………………………..85%

Sulfadimethoxypyrimidine ……………………………………………………………….67%

Benzene Sulfapyridine ……………………………………………………………………..62%

Sulfapyridine……………………………………………………………………………………55%

Sulfamerazine………………………………………………………………………………….52%

Sulfamethizole…………………………………………………………………………………49%

Sulfachlorpyridazine ……………………………………………………………………..29.6%

Sulfamethazine………………………………………………………………………………..13%

Percent Recovery, %

Tissue sample ………………………………………………………………………….. 80±10%

Serum sample………………………………………………………………………….. 90±15%

Precision (CV %):

Intra-lab assay: CV%≤10%

Kit ContentsQuantityStorage
Antigen coated plate96T2-8°C
5 vials of Standard1mL each2-8°C
High concentration standard (1ppm)1mL2-8°C
Enzyme Conjugate6mL2-8°C
Antibody Solution9mL2-8°C
Substrate A6mL2-8°C
Substrate B6mL2-8°C
Concentrated Sample Diluting Buffer I (10× )45mL2-8°C
Concentrated Sample Diluting Buffer II (2× )45mL
Concentrated Wash Buffer (10× )40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

 

 

Bestsellers

Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Aflatoxin M1 Immunoaffinity Column

Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

Aflatoxin M1 Qualitative Lateral Flow Rapid Test Kit

Aflatoxin M1 Qualitative Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of aflatoxin M1 in raw cow’s milk. This test is designed for rapid field use or daily control in laboratories.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Tetracyclines ELISA Test Kit for Animal Tissue, Seafood and Egg

Qualitative or quantitative analysis of tetracycline, oxytetracycline, aureomycin, doxycycline in animal tissue, seafood and egg, etc.Meizheng Bio-Tech Company Overview. PDF

 

Back to Top
Product has been added to your cart