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    Vitamin B12 ELISA Test Kit for Milk Powder

    Qualitative or quantitative analysis of Vitamin B12 in milk powder.

    Brochure-Dairy Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Vitamin B12, also called cobalamin, is a water-soluble vitamin that has a key role in the normal functioning of the brain and nervous system via the synthesis of myelin (myelinogenesis), and the formation of red blood cells. It is one of eight B vitamins.

    HPLC method is now widely used in the detection of Vitamin B12, but its high cost and complex operation make it unacceptable.

    The Vitamin B12 (VB12) ELISA Test Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.

    Use Principles: 

    Vitamin B12 (VB12) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Vitamin B12 antigen is precoated on the wells. The precoated antigen compete the Vitamin B12 antibody (antibody solution) with Vitamin B12 in the sample, Vitamin B12 antibody binds to the Vitamin B12 enzyme conjugate. After the color development of TMB substrate, the absorbance value of the sample is negatively correlated with the Vitamin B12 contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Vitamin B12 in the sample can be calculated.

    ManufacturerMeizheng
    Order No.SC0071
    AnalyteVitamin B12
    Sample TypeMilk powder
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityVitamin B12……………………………………100%
     Calculate ResultAnalyze the result by using the software
    Sensitivity0.25 ppb
    Limit of detectionMilk powder……………………………3.75ppb
    Percent Recovery, %100±20%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96T
    6 vials of standard1mL each
    Enzyme Conjugate12mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2
    Component96 wells
    Antigen coated plate96T

    Note: The concentrations of the standards are 0ppb, 0.25ppb, 0.75ppb, 2.25ppb, 6.75ppb and 20.25ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm)

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Rubber suction bulb

    ---- Centrifuge tube: 50mL

    ---- Micropipette: Single channel pipettte (10µL-100µL, 100µL-1000µL)

    Multi-channel pipette (30µL-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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