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Wheat and Flour Mycotoxin Deoxynivalenol (DON) ELISA Test Kit

The Deoxynivalenol ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Deoxynivalenol in rice flour, wheat, barley, corn, flour, oats etc.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Deoxynivalenol is known as vomitoxin or DON. It is a potent fungal toxin to affect crops grown in cool and moist conditions. Fusarium graminearum is the principal DON-producing fungus in grains but Fusarium culmorum is often involved as well especially in certain geographical areas of the world. Deoxynivalenol is a known immunosuppressant and may cause kidney problems. Consuming DON-contaminated grain can cause vomiting in humans and animals. Deoxynivalenol is formed by the Fusarium species and mainly affects corn, wheat, oats and barley.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM0896
AnalyteDeoxynivalenol
Sample TypeFlour, Wheat, Barley, Corn, Flour, Oats, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityDON…………………………………………100%
3-AcDON……………………………………<70%
15-AcDON……………………………..……<1%
Oxynivalenol …………………………………<1%
Result InterpretationAnalyze the result by using the software
Sensitivity4 ppb
Limit of detection100ppb
Percent Recovery  110±15%
Intra-lab assayCV%≤10%

 Provided Materials and Reagent:

Component96 wells
Antigen coated plate12 X 8 wells
5 vials of Standard1mL each
DON Enzyme Conjugate6mL
DON Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Stop Solution6mL
Concentrated Wash Solution (10X)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

Note: The concentrations of the standards are 0µg/kg, 4µg/kg, 20µg/kg, 80µg/kg and 200µg/kg respectively.

Materials Required But Not Provided

Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Oscillator/ Vortex

—- Centrifuge (or funnel)

—- Balance (sensibility: 0.01g)

—- Pipette: 10mL

—- Centrifuge tube: 50mL (or triangular flask with stopper)

—- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)

Reagent:

—- Deionized water

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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