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    Aflatoxin M1 ELISA Test Kit for Milk

    The Aflatoxin M1 ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Aflatoxin M1 concentrations in liquid milk, fermented milk, milk power, cheese, etc.

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    Background:

    Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cotton seed and human blood.

    Aflatoxin B1 is a potent human carcinogen and may contribute to human liver cancer.

    Aflatoxin M1 is one of Aflatoxin with similar structure  compounds. On damp-heat areas, the occurrence of Aflatoxin is highest in food and feed. Aflatoxin M1 with quite stable physical and chemistry properties, is not destroyed by pasteurization. When the mammal animal ingests food or feed containing Aflatoxin B1, it can be converted to the Aflatoxin M1 by hydroxylation. The main harm of Aflatoxin M1 is carcinogenicity and mutagenicity, destroying human and animal liver tissue, resulting in hepatocarcinoma and even death.

    Use Principles:

    The Aflatoxin M1 kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin M1 antigen is precoated on the wells. The aflatoxin M1 in the sample compete the aflatoxin M1 antibody (antibody solution) with aflatoxin M1 antigen on the well, while the M1 antibody combines with the enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Aflatoxin M1 concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.SC0354
    AnalyteAflatoxin M1
    Sample TypeRaw milk, Milk products
    MethodologyLiquid milk, fermented milk, milk power, cheese, etc.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross Reactivity

     

    AFM1 100%

    AFM2 15.5%

    Calculate ResultAnalyze the result by using the software
    Sensitivity 5ppt
    Limit of detection0.005 ppb
    Percent Recovery, %100±30%
    Precision (CV %):Intra-lab assay:  CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 x 8 wells each
    5 vials of Standard3mL each
    AFM1 Enzyme Conjugate12mL
    AFM1 Antibody solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated wash buffer (10x)40mL
    Concentrated sample diluting buffer (8x)50mLx2
    Test report1
    Plate cover sheet2
    Package insert1

    Note: The concentrations of the standards are 0ng/kg, 5ng/kg, 15ng/kg, 45ng/kg and 135ng/kg respectively.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex

    ---- Centrifuge /funnel

    ---- Balance (sensibility: 0.01g)

    ----Micropipette:Single channel pipet (10µL-100µL, 100µL-1000µL). Multi-channel pipet (30-300µL)

    ----Nitrogen evaporator

    ---- Graduated pipette (10mL)

    ---- Centrifuge tube

    2. Reagent:

    ---- Deionized water

    ---- Methanol (AR)

    -----Sodium chloride (AR)

    ---- Concentrated sulfuric acid (18 mol/L)

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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