Spore-Based Test Kits adopt the principle of microbial inhibition. The microwell plate is filled with a medium containing Geobacillus stearothermophilus spores and the pH indicator. When cultured at 65℃, the spores germinate, the bacteria grow, and then metabolize to produce acid. The pH value decreases and the indicator changes from the original purple to yellow. If the antibiotics exist in the added milk and dairy products, the antibiotics will inhibit bacterial growth, thus no acid is produced, the indicator does not change the color, and the original purple is maintained; if the antibiotics do not exist in the added milk and dairy products or the concentration of antibiotics is below the detection limit, the spores germinate, metabolize and product acid, and the medium turns yellow.
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