Search

In Stock

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.

Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Overview:

Aflatoxins are toxic metabolites of a class of fungi (such as Aspergillus flavus and Aspergillus parasiticus). They are highly carcinogenic and are found mainly in grains, peanuts, nuts, cottonseeds, animal feed, vegetable oils, as well as animal tissues and blood. Among them, Aflatoxin B1 (AFT B1) ranks first in terms of toxicity, carcinogenicity and frequency of contamination.

 Principle:

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the aflatoxin in the sample is extracted, filtered, and diluted, and then passed slowly through the aflatoxin immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Aflatoxin is then eluted with methanol and injected into an analytical instrument for detection.

ManufacturerMeizheng
Order No.HCM0125B
Sample TypeLiquid milk, yogurt, Milk powder, special dietary foods, Cheese, Grain and oil, nuts, feed, herbs, tea, condiments, dairy products, etc.
Packaging25 Tests
Column Capacity300 ng
Volume of Column3 ml
StorageThe storage temperature is 2-8˚C.
Shelf lifeThe validity for this kit is 18 months
Lead timeShipped within 5-12 working days.

Provided Materials and Reagent

  1. Aflatoxin immunoaffinity columns: 25 T
  2. 1 instruction manual.

Materials Required But Not Provided

Equipment

  • HPLC/LC-MS/MS
  • Nitrogen gas evaporator apparatus
  • Nitrogen gas tank and pressure regulator
  • Air-pressure controller bracket
  • Air pump
  • Balance with 0.001g readability
  • High-speed freezing centrifuge (can be set to 10°C, 7, 500 RPM)
  • Centrifuge tube:50-mL
  • Constant temperature incubator or water bath (can be set to 35–37℃)
  • High-speed homogenizer (maximum speed> 10,000 RPM) or shaker
  • Grinder
  • pH meter (or pH test paper)
  • Sieving screen: 2mm
  • Graduated cylinder: 100 mL/10 mL
  • Funnel: 50 mL
  • Syringe: 10 mL/20 mL
  • Pipette: 1 mL and pipette tips
  • Homogenization flask (or 250-mL conical flask with pestle)
  • Vias and tubes
  • Rapid qualitative filter paper
  • Microfiber filter paper (e.g. Whatman 934-AH)
  • Column holder and syringe connector plug (for use with immunoaffinity columns)

Reagents:

  • Methanol (CH3OH): Analytical Grade
  • Acetonitrile (CH3CN): Chromatography Grade
  • Sodium chloride (NaCl): Analytical Grade
  • Hydrochloric acid (HCl): Analytical Grade
  • Sodium hydroxide (NaOH): Analytical Grade
  • Potassium chloride (KCl): Analytical Grade
  • Potassium dihydrogen phosphate (KH2PO4): Analytical Grade
  • Disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O): Analytical Grade
  • Distilled or deionized water

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

Bestsellers

Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

Aflatoxin M1 Immunoaffinity Column

Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

Milk Ansamycins Rapid Test Kit

The Ansamycins Rapid Test Kit is a Lateral Flow assay that determines the presence of ansamycins residues in raw milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Total Aflatoxins Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a qualitative level for the presence of total aflatoxins and is intended for use in grains, feed materials and feed products. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

 

Back to Top
Product has been added to your cart