Background:
The quinolones are a family of synthetic broad-spectrum antibiotics. The majority of quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.
The Quinolones ELISA Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.
Use Principles：
Quinolones ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.
| Manufacturer | Meizheng |
| Order No. | SC0039 |
| Analyte | Quinolones |
| Sample Type | Honey, milk, milk powder, etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Ciprofloxacin (CIF) 100% Enrofloxacin (ENR) 200% Norfloxacin (NOR) 150% Ofloxacin (OFL) 60% Danofloxain (DAN) 60% Sarafloxacin (SAR) 50% Marbolxacin (MAR) 12% Enoxacin (ENO) 85% Pefloxacin (PEF) 61% Lomefloxacin (LOM) 73% Enoxacin (ENX) 110% Difloxacin (DIF) 50% Amifloxacin (AMI) 75% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.5ppb |
| Limit of detection | Honey, milk powder 2ppb Milk 4ppb |
| Percent Recovery, % | Milk and honey 100±20% Milk powder…………………………………………………………….90±30% |
| Precision (CV %): | Intra-lab assay: CV<10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of standard | 1ml each |
| High Concentration Standard (1ppm) | 1ml |
| Auxiliary extracting agent | 3ml |
| QNs Enzyme conjugate | 6ml |
| QNs Antibody Solution | 6ml |
| Substrate A | 6ml |
| Substrate B | 6ml |
| Concentrated Wash Buffer (10×) | 40ml |
| Concentrated sample diluting buffer | 40ml |
| IFU | 1 |
| Plate cover sheet | 2 |
| Testing Report | 1 |
Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb, 40.5ppb.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Vortex
—- Incubator (optional if the room temperature can reach 25℃)
—- Rotary evaporator/nitrogen evaporator
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Transfer pipette
—- Centrifuge tube: 10ml
—- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL), Multi-channel pipette (30µL -300µL)
- Reagent:
—- Methanol (AR)
—- Dichloromethane (AR)
—- N-hexane (AR)
—- Sodium sulphate anhydrous (AR)
—- Sodium carbonate (AR)
—- Sodium hydrogen carbonate (AR)
—- Ethyl acetate (AR)
—- Acetonitrile (AR)
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
