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    Antibiotic Quinolones ELISA Test Kit for Honey and Dairy

    Qualitative or quantitative analysis of quinolones in honey, milk, milk powder, etc.

    Brochure-Dairy Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    The quinolones are a family of synthetic broad-spectrum antibiotics. The majority of quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.

    The Quinolones ELISA Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.

    Use Principles:

    Quinolones ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.SC0039
    AnalyteQuinolones
    Sample TypeHoney, milk, milk powder, etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityCiprofloxacin (CIF) 100%
    Enrofloxacin (ENR) 200%
    Norfloxacin (NOR) 150%
    Ofloxacin (OFL) 60%
    Danofloxain (DAN) 60%
    Sarafloxacin (SAR) 50%
    Marbolxacin (MAR) 12%
    Enoxacin (ENO) 85%
    Pefloxacin (PEF) 61%
    Lomefloxacin (LOM) 73%
    Enoxacin (ENX) 110%
    Difloxacin (DIF) 50%
    Amifloxacin (AMI) 75%
    Calculate ResultAnalyze the result by using the software
    Sensitivity 0.5ppb
    Limit of detectionHoney, milk powder 2ppb
    Milk 4ppb
    Percent Recovery, %Milk and honey 100±20%
    Milk powder......................................................................90±30%
    Precision (CV %):Intra-lab assay: CV<10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1ml each
    High Concentration Standard (1ppm)1ml
    Auxiliary extracting agent3ml
    QNs Enzyme conjugate6ml
    QNs Antibody Solution6ml
    Substrate A6ml
    Substrate B6ml
    Concentrated Wash Buffer (10×)40ml
    Concentrated sample diluting buffer40ml
    IFU1
    Plate cover sheet2
    Testing Report1

     

    Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb, 40.5ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex

    ---- Incubator (optional if the room temperature can reach 25℃)

    ---- Rotary evaporator/nitrogen evaporator

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette

    ---- Centrifuge tube: 10ml

    ---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL), Multi-channel pipette (30µL -300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Dichloromethane (AR)

    ---- N-hexane (AR)

    ---- Sodium sulphate anhydrous (AR)

    ---- Sodium carbonate (AR)

    ---- Sodium hydrogen carbonate (AR)

    ---- Ethyl acetate (AR)

    ---- Acetonitrile (AR)

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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