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    Chloramphenicol ELISA Test Kit for Meat, Seafood, Liver, Egg, Swine Urine and Feed

    The Chloramphenicol ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of Chloramphenicol in samples of poultry tissues, feed, swine urine, eggs and etc.

    Summary

    Chloramphenicol (CAP) is a widely used antibiotic, but has serious side effects, which can cause aplastic anemia, agranulocytosis and other diseases.

    The detection method is sensitive, fast, simple and specific, only need 45minu for operating and a small amount of sampling.

    Order No.: ME01AER

    Sensitivity: 0.02 ppb

    Package: 96 T

    Sample: Meat, Seafood, Liver, Egg, Swine urine, Feeds

    Use Principles

    The Chloramphenicol ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Chloramphenicol in the sample, anti-Chloramphenicol antibody binds to the HRP enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Chloramphenicol concentration of the samples is derived.

    Cross-reactivity

    Chloramphenicol: 100%

    Chloramphenicol Alkali: 0.5%

    Methylchloramphenicol: ≤0.05%

    Florfenicol:<0.01%

    Flurfenicomide:<0.1%

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex Mixer

    ---- Constant temperature incubator

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ----Water bath

    ---- Homogenizer

    ---- Nitrogen evaporator

    ---- Graduated suction pipet (10ml)

    ---- Micropipet: Single channel pipet (10µl-100µl, 100µl-1000µl)

    Multi-channel pipet (30µl-300µl)

    ---- Centrifuge tube (50ml, 10ml or 7ml)

    1. Reagent:

    ---- Deionized water

    ----Ethyl acetate (analytically pure)

    -----N-hexane (analytically pure)

    ---- Concentrated sulfuric acid (18 mol/L)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of Standard1ml each
    High concentration standard (100ppb)1ml each
    CAP Enzyme Conjugate6ml
    CAP Antibody Solution6ml
    Substrate A6ml
    Substrate B6ml
    Concentrated Wash Buffer (10X)40ml
    Concentrated sample diluting  buffer40ml
    Concentrated sample extracting buffer30ml
    IFU1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb respectively.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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