Background:
Oxytetracycline is a kind of antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. Oxytetracycline have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.
The Oxytetracycline ELISA Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.
Use Principles：
Oxytetracycline ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The oxytetracycline antigen is precoated on the wells. The precoated antigen compete the oxytetracycline antibody (antibody solution) with sulfonamides in the sample, anti- oxytetracycline antibody binds to the oxytetracycline -HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the oxytetracycline concentration of the samples is derived.
| Manufacturer | Meizheng |
| Order No. | SC0052 |
| Analyte | Oxytetracycline |
| Sample Type | Honey, milk, etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Oxytetracycline 100% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.2ppb |
| Limit of detection | 2ppb |
| Percent Recovery, % | 90±15% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 96T |
| 6 vials of standard | 2mL each |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Concentrated sample diluting buffer | 40mL |
| IFU | 1 |
| Testing Report | 1 |
| Plate cover sheet | 2 |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.
Materials Required But Not Provided
1.Devices:
—- Microwell plate Reader (450nm/630nm)
—- Vortex
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Transfer pipette: 10mL
—- Rubber bulb
—- Centrifuge tube: 10mL, 50mL
—- Micropipette: Single channel pipette (10μL-100μL, 100μL-1000μL)
Multi-channel pipette (30μL -300μL)
- Reagent:
—- Deionized water
—- Concentrated sulfuric acid (18mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
