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Dairy and Honey Oxytetracycline ELISA Test Kit

Qualitative or quantitative analysis of oxytetracycline residues in milk, honey, etc.

Brochure-Dairy Test. PDF

Brochure-Honey Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Oxytetracycline is a kind of antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. Oxytetracycline have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

The Oxytetracycline ELISA Kit produced by Meizheng significantly reduces both the testing cost and the operating difficulties.

Use Principles:

Oxytetracycline ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The oxytetracycline antigen is precoated on the wells. The precoated antigen compete the oxytetracycline antibody (antibody solution) with sulfonamides in the sample, anti- oxytetracycline antibody binds to the oxytetracycline -HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the oxytetracycline concentration of the samples is derived.

ManufacturerMeizheng
Order No.SC0052
AnalyteOxytetracycline
Sample TypeHoney, milk, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityOxytetracycline 100%
 Calculate ResultAnalyze the result by using the software
Sensitivity   0.2ppb
Limit of detection2ppb
Percent Recovery, %90±15%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate96T
6 vials of standard2mL each
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated sample diluting buffer40mL
IFU1
Testing Report1
Plate cover sheet2
Antibody Solution6mL
Substrate A6mL

Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.

Materials Required But Not Provided

1.Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Rubber bulb

—- Centrifuge tube: 10mL, 50mL

—- Micropipette: Single channel pipette (10μL-100μL, 100μL-1000μL)

Multi-channel pipette (30μL -300μL)

  1. Reagent:

—- Deionized water

—- Concentrated sulfuric acid (18mol/L)

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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