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    Dairy Antibiotics Spore High-throughput Test Kit

    DASH (Dairy Antibiotics Spore High-throughput) Test Kit is applicable to qualitative analysis of antibiotic residues in raw milk (including cattle, sheep, and goats).

    Flyer – DASH Test Kit. pdf

    Brochure-Dairy Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    ManufacturerMeizheng
    Order No.WC0006
    AnalyteAntibiotic residues
    Sample TypeRaw milk (including cattle, sheep, and goats).
    MethodologyMicrobial inhibition
    Packaging96 Tests
    Storage4-8℃
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.

    Principle of Test

    This kit adopts the principle of microbial inhibition. The microwell plate is filled with a medium containing Geobacillus stearothermophilus spores and the pH indicator. When cultured at 65℃, the spores germinate, the bacteria grow, and then metabolize to produce acid. The pH value decreases and the indicator changes from the original purple to yellow. If the antibiotics exist in the added milk and dairy products, the antibiotics will inhibit bacterial growth, thus no acid is produced, the indicator does not change the color, and the original purple is maintained; if the antibiotics do not exist in the added milk and dairy products or the concentration of antibiotics is below the detection limit, the spores germinate, metabolize and product acid, and the medium turns yellow.

    Detection Limit

    AntibioticDetection Limit (μg/L)
    Beta-lactams
    Penicillin G2
    Ampicillin2-3
    Amoxicillin2-3
    Nafcillin5
    Cloxacillin10-15
    Oxacillin6
    Dicloxacillin10-15
    Cefacetrile20
    Cefalexin45
    Cefalonium5-6
    Cefoperazone30
    Ceftiofur50
    Cefquinome75
    Cefapirin2.5-5
    Cefazolin10-15
    Tetracyclines
    Chlortetracycline200
    Doxycycline75-100
    Oxytetracycline100-150
    Tetracycline100-150
    Aminoglycosides
    Gentamicin100
    Kanamycin2500
    Neomycin200-300
    Spectinomycin>300
    Streptomycin1100-1500
    Dihydrostreptomycin700-1000
    Sulfonamides
    Sulfamethazine50-100
    Sulfadimethoxine25-50
    Sulfamerazine100
    Sulfathiazole40
    Sulfadiazine50
    Sulfadoxine80-120
    Sulfamethoxazole<25
    Sulfamethoxpyridazine50-100
    Sulfisoxazole50
    Sulfachloropyridazine25-50
    Sulfaphenazole25-50
    Sulfamethoxydiazine50-100
    Sulfabenzamide100
    Sulfaclozine100
    Sulfapyrazole50
    Sulfamethoxypyrazine25-50
    Sulfisomidine50-100
    Sulfamonomethoxine50
    Sulfapyridine100
    Sulfaquinoxaline50
    Sulfamethazole100
    Sulfamethizole50-100
    Macrolides
    Erythromycin100-150
    Spiramycin400-800
    Tilmcosin100
    Tylosin40-80
    Quinolones
    Ciprofloxacin1000-1500
    Danofloxacin1000-2000
    Marbofloxacin>1000
    Various
    Chloramphenicol2500
    Rifaximin60-100
    Pirlimycin200
    Thiamphenicol>1000
    Trimethoprim100
    Lincomycin300-500

    Reagents and Materials Supplied

    Detachable microwell plate3 pieces

    (96 independent tests each piece)

    Cover film (Aluminum foil)3
    User manual1
    Colorimetric card1

    Materials required but not supplied

    ---- Stable 65°C heating equipment (water bath or dry heater)

    ---- Single channel micropipette (2.5-100µL)

    ---- Negative control: milk without antibiotics

    ---- Positive control: milk containing 10mg/L Penicillin G or 10mg/L Ampicillin

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

     

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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