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    Dairy Hormones Zeranol ELISA Test Kit

    Qualitative or quantitative analysis of zeranol in raw milk, tank milk, and finished milk.

    Order No.: SC0065

    【Summary】

    Zeranol (ZAL) is an ideal skin-buried weight-increasing agent. It is a microbial metabolite and is a non-sterol, non-hormonal compound. “Zeranol” has an estrogen-like effect and is banned in most developed countries. Zeranol and its metabolites have the biological activity of estrogens and have inhibitory effects on gonadotropin-binding receptors and in vitro liver hormone-binding receptors. Residues of estrogens can cause sexual hormonal disorders and affect the normal development of secondary sexual characteristics, which may cause cancer under the induction of external conditions.

    【Use Principles

    Zeranol ELISA Test Kit is an indirect competitive enzyme-linked immunoassay. The zeranol antigen is precoated on the wells. The precoated antigen compete the zeranol antibody (antibody solution) with zeranol in the sample, anti-zeranol antibody binds to the zeranol-HRP enzyme conjugate. Then pipette the substrate solution into the wells to convert the color into a blue product.

    The color of unknown samples is compared to the color of the standards and the Zeranol concentration of the samples is derived. The absorbance is inversely proportional to the Zeranol concentration in the sample.

    【Cross Reactivity】

    α-zeranol 100%

    β-zeranol 100%

    α-zearalenol 133%

    β-zearalenol 13%

    Zearalenone  111%

    Zearalanone  316%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Vortex

    ---- Constant Temperature Incubator

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component 96 wells
    Antigen coated plate12 × 8 wells
    6 vials of standard1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer30mL
    Package Insert1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0µg/kg, 0.1µg/kg, 0.3µg/kg, 0.9µg/kg, 2.7µg/kg, and 8.1µg/kg.

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