Zeranol (ZAL) is an ideal skin-buried weight-increasing agent. It is a microbial metabolite and is a non-sterol, non-hormonal compound. “Zeranol” has an estrogen-like effect and is banned in most developed countries. Zeranol and its metabolites have the biological activity of estrogens and have inhibitory effects on gonadotropin-binding receptors and in vitro liver hormone-binding receptors. Residues of estrogens can cause sexual hormonal disorders and affect the normal development of secondary sexual characteristics, which may cause cancer under the induction of external conditions.
Zeranol ELISA Test Kit is an indirect competitive enzyme-linked immunoassay. The zeranol antigen is precoated on the wells. The precoated antigen compete the zeranol antibody (antibody solution) with zeranol in the sample, anti-zeranol antibody binds to the zeranol-HRP enzyme conjugate. Then pipette the substrate solution into the wells to convert the color into a blue product.
The color of unknown samples is compared to the color of the standards and the Zeranol concentration of the samples is derived. The absorbance is inversely proportional to the Zeranol concentration in the sample.
【Materials Required But Not Provided】
---- Microwell Plate Reader (450nm/630nm)
---- Constant Temperature Incubator
---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
---- Deionized water
---- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||12 × 8 wells|
|6 vials of standard||1mL each|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer||30mL|
|Plate cover sheet||2|
Note: The concentrations of the standards are 0µg/kg, 0.1µg/kg, 0.3µg/kg, 0.9µg/kg, 2.7µg/kg, and 8.1µg/kg.