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    Dairy Immunoaffinity Column for Folic Acid IAC Column

    The immunoaffinity column can selectively adsorb folic acid from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.

    Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

    Order No.: QC0085

    Product Properties: 500ng

    Specifications: 3mL, 50T

    Matrix: Milk powder,Dairy products

    1. Overview:

    Folic acid is an important type of B vitamin that is normally found in foods. It helps your body produce and maintain new cells, and also helps prevent changes to DNA that may lead to cancer. As a medication, folic acid is used to treat folic acid deficiency and certain types of anemia (lack of red blood cells) caused by folic acid deficiency.

    1. Principle:

    The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and folic acid in the sample is extracted, filtered, and diluted, and then passed slowly through the folic acid immunoaffinity column. The folic acid binds to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Folic acid then eluted and injected into an analytical instrument for detection.

    2. Components of the kit:

    Each kit contains folic acid immunoaffinity columns and 1 instruction manual.

    3. Necessary items not provided in the box:

    • Equipment
    • HPLC /LC-MS(LC-MS/MS)
    • Nitrogen gas evaporatorapparatus
    • Nitrogen gas tank and pressureregulator
    • Air-pressure controller bracket
    • Air pump
    • Balance with 0.01greadability
    • Ultrasoniccleaner
    • High-speed freezing centrifuge (can be set to 10℃, 6000RPM)
    • Centrifugetube:50-mL
    • Constant temperature incubator or water bath (can be set to 35–37℃)
    • High-speed homogenizer (maximum speed> 10,000 RPM) or shaker
    • Grinder
    • pH meter (or pH testpaper)
    • Volumetric flask: 100 mL/50mL/250mL/500mL/1000mL
    • Syringe: 10 mL/20mL
    • Homogenization flask (or 250-mL conical flask withpestle)
    • Vials andtubes
    • Pipette: 1 mL /10 mL and pipettetips
    • Column holder and syringe connector plug (for use with immunoaffinitycolumns)

    3.2 Reagents

    • Methanol (CH3OH): Chromatographygrade
    • Sodium hydroxide (NaOH): AnalyticalGrade
    • Sodium chloride (NaCl): AnalyticalGrade
    • Hydrochloric acid (HCl): ChromatographyGrade
    • Potassium dihydrogen phosphate (KH2PO4): AnalyticalGrade
    • Dipotassium phosphate (K2HPO4): AnalyticalGrade
    • Sodium carbonate (Na₂CO₃): AnalyticalGrade
    • Sodium bicarbonate (NaHCO₃): AnalyticalGrade
    • Pancreatin:≥1U/mg
    • L-ascorbic acid sodium salt(C6H7O6Na): AnalyticalGrade
    • Distilled/deionizedwater

    4. Note:

    • Allow the immunoaffinity column to return to room temperature (22 to 25°C) before
    • The affinity column should be stored at 2 to 8°C, do not
    • Dot use any expired immunoaffinity
    • Column capacity:500 ng, when the content of the folic acid in the sample divided by the dilution factor is higher than the column capacity, it is necessary to reduce the volume of the sample solution appropriately, and
    • The pH of the loading solution of the affinity column should be between 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium

    Keeping the solvent and mobile phase consistent with the instrument detection can eliminate the effect of solvent effects.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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