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    Dairy Qualitative and Quantitative Alpha-lactalbumin ELISA Test Kit

    Qualitative or quantitative analysis of alpha-lactalbumin content in raw milk, pasteurized milk, and finished milk.

    Order No.: SC0162

    【Summary】

    Alpha-lactalbumin is a kind of whey protein with various functions such as regulating milk production, cytolytic activity, inducing cell growth inhibition and apoptosis, helping to relieve stress and improve work performance, and improve sleep, improve abstract visual memory.

    【Principles】

    Alpha-lactalbumin ELISA Test Kit is an indirect competitive enzyme-linked immunoassay. The antibody is pre-coated on the wells, the alpha-lactalbumin in the sample competes with the enzyme conjugated antigen to bind the added alpha-lactalbumin antibody (antibody solution), which is colored by the TMB substrate. The absorbance value of the sample is negatively correlated with the content of alpha-lactalbumin, and the content of alpha-lactalbumin in the sample can be calculated by comparing with the standard curve and multiplying the corresponding dilution factor.

    Cross-reactivity

    α-lactalbumin…………………………………….………….100%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Constant Temperature Incubator

    ---- Centrifuge tube: 7mL

    ---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

    Multi-channel pipette (30-300μL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component 96 wells
    Antibody coated plate12 × 8 wells
    6 vials of standard1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Package Insert1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0mg/kg, 0.1mg/kg, 0.3mg/kg, 0.9mg/kg, 2.7mg/kg and 8.1mg/kg.

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