Alpha-lactalbumin is a kind of whey protein with various functions such as regulating milk production, cytolytic activity, inducing cell growth inhibition and apoptosis, helping to relieve stress and improve work performance, and improve sleep, improve abstract visual memory.
Alpha-lactalbumin ELISA Test Kit is an indirect competitive enzyme-linked immunoassay. The antibody is pre-coated on the wells, the alpha-lactalbumin in the sample competes with the enzyme conjugated antigen to bind the added alpha-lactalbumin antibody (antibody solution), which is colored by the TMB substrate. The absorbance value of the sample is negatively correlated with the content of alpha-lactalbumin, and the content of alpha-lactalbumin in the sample can be calculated by comparing with the standard curve and multiplying the corresponding dilution factor.
【Materials Required But Not Provided】
---- Microwell Plate Reader (450nm/630nm)
---- Oscillator / Vortex
---- Constant Temperature Incubator
---- Centrifuge tube: 7mL
---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)
Multi-channel pipette (30-300μL)
---- Deionized water
---- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antibody coated plate||12 × 8 wells|
|6 vials of standard||1mL each|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer||40mL|
|Plate cover sheet||2|
Note: The concentrations of the standards are 0mg/kg, 0.1mg/kg, 0.3mg/kg, 0.9mg/kg, 2.7mg/kg and 8.1mg/kg.