Dexamethasone, also known as flumethasone, flumazetone, desamethasone, is a glucocorticoid. Its derivatives include hydrocortisone and prednisone. Dexamethasone has anti-inflammatory, anti-allergic, anti-rheumatic, and immunosuppressive effects. However, this hormone remains in the food and has potential harm to humans. Long-term consumption of food left over from veterinary drugs will cause veterinary drugs to accumulate in the body. When its concentration reaches a certain amount, it will cause various acute or chronic poisoning to the human body.
Dexamethasone is a hormonal drug that can disrupt hormone secretion in the body, reduce the body’s immunity, cause muscle atrophy and weakness, osteoporosis and growth retardation, and other direct harms. It can also cause fever, weakness, mental depression, loss of appetite, symptoms such as decreased blood sugar and blood pressure. Countries such as the European Union have banned dexamethasone as a growth promoter for livestock and used it in animal husbandry.
Dexamethasone ELISA Test Kit is an indirect competitive enzyme-linked immunoassay. The dexamethasone antigen is precoated on the wells. The precoated antigen compete the dexamethasone antibody (antibody solution) with dexamethasone in the sample, anti- dexamethasone antibody binds to the dexamethasone-HRP enzyme conjugate. Then pipette the substrate solution into the wells to convert the color into a blue product.
The color of unknown samples is compared to the color of the standards and the dexamethasone concentration of the samples is derived. The absorbance is inversely proportional to the dexamethasone concentration in the sample.
【Materials Required But Not Provided】
—- Microwell Plate Reader (450nm/630nm)
—- Constant Temperature Incubator
—- Balance (sensibility: 0.01g)
—- Graduated pipette: 2mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
—- Deionized water
—- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||12 × 8 wells|
|5 vials of standard||1.5 mL each|
|High Concentration Standards (100ppb)||1mL each|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer Ⅰ||15mL|
|Concentrated Sample Diluting Buffer Ⅱ||50mL x 2|
|Plate cover sheet||2|
Note: The concentrations of the standards are 0µg/kg, 0.05µg/kg, 0.15µg/kg, 0.45µg/kg, 1.35µg/kg.