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    Dairy Staphylococcus aureus Enterotoxin Typing ELISA Test Kits

    It can qualitatively detect the content of Staphylococcus aureus enterotoxin A, B, C, D, and E in raw milk, finished milk, and fermented milk sample.

    Flyer-Staphylococcus aureus Enterotoxin ELISA Test Kit. pdf

    Brochure-Dairy Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    ManufacturerMeizheng
    Order No.LR50511
    AnalyteStaphylococcus aureus enterotoxin
    Sample TypeRaw milk, finished milk, and fermented milk sample.
    MethodologyDouble antibody sandwich ELISA method
    Packaging96 Tests
    StorageT  The storage temperature for the kits is 2-8℃.
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.

     Introduction

    Enterotoxin (SE) is a pathogenic substance that causes Staphylococcus aureus food poisoning. According to its antigenicity, it can be divided into 8 serotypes A-E and G-I, of which SEA is the most common. Enterotoxins in food are not easily inactivated during processing and can cause symptoms such as vomiting and diarrhea. In severe cases, they can cause multiple organ dysfunction and be life-threatening. This kit is a new generation of Staphylococcus aureus total enterotoxin (SET) detection product using ELISA, which can minimize operating errors and work intensity.

    Principle of Test

    This kit adopts double antibody sandwich ELISA method. Precoat the total amount of Staphylococcus aureus enterotoxin antibody on the microplate. Both the total amount of precoated enterotoxin antibody and detection antibody bind to the enterotoxin in the sample at the same time. And then the complex combines with the HRP-conjugate to form an antibody-enterotoxin-antibody-enzyme-HRP-conjugate, which can be colored by TMB substrate. The absorbance of the sample is positively correlated with the content of Staphylococcus aureus enterotoxin.

     Parameters

    Sensitivity: 0.25-1μg/kg

    Limit of Detection: see the table below

    SampleLOD (μg/kg)
    SEASEBSECSEDSEE
    Raw milk, finished milk0.50.250.250.251
    Fermented milk10.50.50.52

    Precision (CV %) Intra-lab assay: CV<10%

    Materials Required but Not Provided

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Vortex

    ---- Constant Temperature Incubator

    ---- Refrigerated centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Centrifuge tube: 2mL, 5mL, 7mL, 50mL

    ----Micropipette: Single channel pipet (10-100µL, 100-1000µL) Multi-channel pipet (30µL -300µL)

    2. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    ---- 1M sodium hydroxide (NaOH)

    Kit components

    Substrate B6mL
    Positive control3mL
    Concentrated Wash Buffer (10×)40mL × 2
    Package insert1
    Plate cover sheet2
    Testing Report1
    Component96 wells
    Antibody coated plate12 × 8 wells
    SETs Antibody Solution12mL
    SETs Enzyme Conjugate12mL
    Substrate A6mL

    Solution Preparation

    1.WashBuffer

    Concentrated Wash Buffer (10×) and deionized water V: V=1:9 mix well.

    2. Stop Solution

    Add 10mL of 18M concentrated sulfuric acid (H2SO4) to 80mL deionized water slowly with stirring and mix well for use. The solution could be stored at room temperature for 2 months.

    3. Sodium Hydroxide Solution

    Prepare 10mL of 1M sodium hydroxide.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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