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    Food Antibiotic Chloramphenicol ELISA Test Kit for Dairy, Honey and Feed

    Qualitative or quantitative analysis of Chloramphenicol in animal tissue (including aquatic product tissue), milk, milk powder, honey, royal jelly, feed, swine urine, etc.

    Brochure-Dairy Test. PDF

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Chloramphenicol is a broad spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.

    ManufacturerMeizheng
    Order No.SC0111
    AnalyteChloramphenicol
    Sample TypeAnimal tissue (including aquatic product tissue), milk, milk powder, honey, royal jelly, feed, swine urine, etc.

     

    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityChloramphenicol……………………………100%
    Chloramphenicol Base…………………..…0.5%
    Methyl Chloramphenicol…………………………≤0.05%
    Florfenicol………………………………..<0.01%
    Florfenicol Amine………………………………………<0.1%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.01ppb
    Limit of detectionTissue, dairy, milk powder, honey, royal jelly.......................................................0.02ppb
    Raw milk, feed………………………..…0.04ppb
    Swine urine………………………………0.1ppb
    Percent Recovery, %80±10%
    Precision (CV %):Intra-lab assay: CV<10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96T
    6 vials of standard1.5mL each
    High Concentration Standard (100ppb)1.5mL
    Enzyme Conjugate6mL
    Antibody Solution4mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Concentrated Milk Powder Sample Extracting Buffer40ml
    Package Insert1
    Plate cover sheet2
    Testing Report1

     Note: The concentrations of the standards are 0ppb, 0.01ppb, 0.03ppb, 0.09ppb, 0.27ppb and 0.81ppb.

    Materials Required But Not Provided

    1 .  Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex mixer

    ---- Centrifuge

    ---- Thermostat incubator

    ---- Balance (sensibility: 0.01g)

    ---- Nitrogen evaporator/Rotatory evaporator

    ---- Water bath

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30-300µL)

    1.  Reagent:

    ---- Ethyl acetate (AR)

    ---- Deionized water

    ---- N-hexane (AR)

    ---- Na2CO3 (AR)

    ---- NaHCO3 (AR)

    ---- Concentrated sulfuric acid (18 mol/L)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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