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Food Antibiotic Chloramphenicol ELISA Test Kit for Dairy, Honey and Feed

Qualitative or quantitative analysis of Chloramphenicol in animal tissue (including aquatic product tissue), milk, milk powder, honey, royal jelly, feed, swine urine, etc.

Brochure-Dairy Test. PDF

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Chloramphenicol is a broad spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.

ManufacturerMeizheng
Order No.SC0111
AnalyteChloramphenicol
Sample TypeAnimal tissue (including aquatic product tissue), milk, milk powder, honey, royal jelly, feed, swine urine, etc.

 

MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityChloramphenicol……………………………100%
Chloramphenicol Base…………………..…0.5%
Methyl Chloramphenicol…………………………≤0.05%
Florfenicol………………………………..<0.01%
Florfenicol Amine………………………………………<0.1%
 Calculate ResultAnalyze the result by using the software
Sensitivity 0.01ppb
Limit of detectionTissue, dairy, milk powder, honey, royal jelly……………………………………………….0.02ppb
Raw milk, feed………………………..…0.04ppb
Swine urine………………………………0.1ppb
Percent Recovery, %80±10%
Precision (CV %):Intra-lab assay: CV<10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate96T
6 vials of standard1.5mL each
High Concentration Standard (100ppb)1.5mL
Enzyme Conjugate6mL
Antibody Solution4mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Concentrated Milk Powder Sample Extracting Buffer40ml
Package Insert1
Plate cover sheet2
Testing Report1

 Note: The concentrations of the standards are 0ppb, 0.01ppb, 0.03ppb, 0.09ppb, 0.27ppb and 0.81ppb.

Materials Required But Not Provided

1 .  Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex mixer

—- Centrifuge

—- Thermostat incubator

—- Balance (sensibility: 0.01g)

—- Nitrogen evaporator/Rotatory evaporator

—- Water bath

—- Graduated pipette: 10mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30-300µL)

  1.  Reagent:

—- Ethyl acetate (AR)

—- Deionized water

—- N-hexane (AR)

—- Na2CO3 (AR)

—- NaHCO3 (AR)

—- Concentrated sulfuric acid (18 mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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