Furaltadone ELISA Test Kit for Meat, Seafood, Liver and Egg

Summary

Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

Order No.:ME04AER

Sensitivity: 0.05 ppb

Package: 96T

Samples: Meat, Seafood, Liver, Egg and Egg Powder, etc.

Use Principles

Furaltadone (AMOZ) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The AMOZ antigen is precoated on the wells. The precoated antigen compete the AMOZ antibody (antibody solution) with AMOZ in the sample, anti-AMOZ antibody binds to the AMOZ-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the AMOZ concentration of the samples is derived.

Cross-reactivity

Furaltadone (AMOZ)…………………………………………………100%

Furazolidone (AOZ)…………………………………………………＜0.1%

Furantoin (AHD)………………………………………………………＜0.1%

Furacilin (SEM)…………………………………………………………＜0.1%

Materials Required But Not Provided

1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex mixer

—- Centrifuge

—- Homogenizer

—- Constant temperature incubator

—- Balance (sensibility: 0.01g)

—- Nitrogen evaporator

—- Water bath

—- Transfer pipette: 10mL

—- Centrifuge tube: 10mL, 50mL,7mL

—- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

Multi-channel pipette (30-300µL)

2. Reagent:

—- Ethyl acetate (AR)

—- Deionized water

—- N-hexane (AR)

—- Methanol (AR)

—- Concentrated hydrochloric acid (AR)

—- NaOH (AR)

—- Dipotassium Hydrogen Phosphate Trihydrate (K2HPO4.3H2O) (AR)

—- Concentrated sulfuric acid (18 mol/L)

Provided Materials and Reagent

Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.