Background:
T2 toxin is a trichothecene mycotoxin initially produced by species of Fusarium. T2 toxin is often found in grains and grain products such as cereals and breads. T2 toxin may act as an anticoagulant at low doses, decreasing fibrinolytic and coagulant signaling pathways. In vivo, T2 toxin alters permeability of the blood-brain barrier (BBB). In the brain and spleen, T2 toxin induces oxidative stress by decreasing levels of glutathione and increasing levels of ROS and lipid peroxidation; it also decreases expression of tissue inhibitor of metalloproteinase 1 (TIMP-1) and increases expression of matrix metalloproteinase 9 (MMP9), TNF-α, IL-1β, and IL-6. In vitro, T2 toxin alters steroidogenesis by inhibiting production of follicular-stimulating hormone (FSH) and progesterone and inhibiting the stimulatory effects of IFG-1.
| Manufacturer | Meizheng |
| Trademark | ToxinFast® |
| Order No. | HEM1396 |
| Analyte | T-2 Toxin |
| Sample Type | Corn, Rice, Beans, Peanut, Oat, Feed And Feed material |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | T-2 Toxin………………………………… …100% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 1 ppb |
| Limit of detection | 50 ppb |
| Percent Recovery, % | 100±20% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent:
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 5 vials of Standard | 1mL each |
| T-2 Enzyme Conjugate | 12mL |
| T-2 Antibody Solution | 12mL |
| Substrate | 10mL*2 |
| Concentrated Wash Buffer (10X) | 40mL |
| Package Insert | 1 |
| Plate Cover Sheet | 2 |
| Testing Report | 1 |
Note: The concentrations of the standards are 0µg/kg, 1µg/kg, 3µg/kg, 9µg/kg and 27µg/kg respectively.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Oscillator/ Vortex
—- Thermostat incubator
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Pipette: 25mL
—- Rubber suction bulb
—- Funnel
—- Triangular flask (with stopper)
—- Centrifuge tube: 10mL, 50mL
—- Micropipet: Single channel pipet (10µl-100µl, 100µl-1000µl)
Multi-channel pipet (30µl-300µl)
- Reagent:
—- Methanol (AR)
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
