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    Grains and Feed T-2 Toxin ELISA Test Kit

    The T-2 ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of T-2 in corn, rice, beans, peanut, oat, feed and feed material.

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    T2 toxin is a trichothecene mycotoxin initially produced by species of Fusarium. T2 toxin is often found in grains and grain products such as cereals and breads. T2 toxin may act as an anticoagulant at low doses, decreasing fibrinolytic and coagulant signaling pathways. In vivo, T2 toxin alters permeability of the blood-brain barrier (BBB). In the brain and spleen, T2 toxin induces oxidative stress by decreasing levels of glutathione and increasing levels of ROS and lipid peroxidation; it also decreases expression of tissue inhibitor of metalloproteinase 1 (TIMP-1) and increases expression of matrix metalloproteinase 9 (MMP9), TNF-α, IL-1β, and IL-6. In vitro, T2 toxin alters steroidogenesis by inhibiting production of follicular-stimulating hormone (FSH) and progesterone and inhibiting the stimulatory effects of IFG-1.

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM1396
    AnalyteT-2 Toxin
    Sample TypeCorn, Rice, Beans, Peanut, Oat, Feed And Feed material
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityT-2 Toxin………………………………… …100%
    Calculate ResultAnalyze the result by using the software
    Sensitivity1 ppb
    Limit of detection50 ppb
    Percent Recovery, %100±20%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent:

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    5 vials of Standard1mL each
    T-2 Enzyme Conjugate12mL
    T-2 Antibody Solution12mL
    Substrate10mL*2
    Concentrated Wash Buffer (10X)40mL
    Package Insert1
    Plate Cover Sheet2
    Testing Report1

    Note: The concentrations of the standards are 0µg/kg, 1µg/kg, 3µg/kg, 9µg/kg and 27µg/kg respectively.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator/ Vortex

    ---- Thermostat incubator

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Pipette: 25mL

    ---- Rubber suction bulb

    ---- Funnel

    ---- Triangular flask (with stopper)

    ---- Centrifuge tube: 10mL, 50mL

    ---- Micropipet: Single channel pipet (10µl-100µl, 100µl-1000µl)

    Multi-channel pipet (30µl-300µl)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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