High Sensitivity Aflatoxin B1 ELISA Test Kit for Dairy, Rice Flour and Feed

【Summary】

Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principal types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in milk, cereals, nuts, cottonseed and human blood.

【Meizheng Bio-Tech Validated Matrixes】

Soybean milk powder, milk powder, rice protein powder, milk, rice flour, yogurt, edible oil, and feed.

【Use Principles】

The Aflatoxin B1 kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin B1 antigen is precoated on the wells. The precoated antigen compete the Aflatoxin B1 antibody (antibody solution) with Aflatoxin B1 in the sample, anti-Aflatoxin B1 antibody binds to the Aflatoxin B1-HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Aflatoxin B1 concentration of the samples is derived.

【Cross Reactivity】

Aflatoxin B1 ………………………………………………….100%

Aflatoxin B2 ………………………………………………….38%

Aflatoxin G1 ………………………………………………….27%

Aflatoxin G2 ………………………………………………….4%

Aflatoxin M1 …………………………………………………20%

Aflatoxin M2 …………………………………………………<1%

【Materials Required But Not Provided】

1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator / Vortex

—- Centrifuge

—- Pipette: 10mL

—- Balance (sensibility: 0.01g)

—- Rubber suction bulb

—- Centrifuge tube: 2mL, 10mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

2. Reagent:

—- Methanol (AR)

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

【Provided Materials and Reagent】

Note: The concentrations of the standard are 0ng/kg, 5ng/kg, 15ng/kg, 45ng/kg, 135ng/kg, 405ng/kg.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.