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    High Sensitivity Aflatoxin B1 ELISA Test Kit for Dairy, Rice Flour and Feed

    The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the quantitative analysis of Aflatoxin B1 in dairy products, rice flour, etc.

    Brochure- Dairy Test Ver.2021-3

    Meizheng Bio-Tech Company Overview-V2022-1 2022.6.14

    48
    days
    10
    hours
    50
    mins
    34
    secs

    $125.00

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM2596
    AnalyteAflatoxin B1
    Sample TypeSoybean milk powder, milk powder, rice protein powder, milk, rice flour, yogurt, edible oil, and feed.
    MethodologyImmunochromatographic Assay
    Packaging96 Tests
    StorageThe kits should be stored in a dry place away from the sunlight. The storage temperature for the kits is 4-30℃. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Limit of detection
    0-0.3μg/kg

    Summary

    Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principal types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in milk, cereals, nuts, cottonseed and human blood.

    Meizheng Bio-Tech Validated Matrixes

    Soybean milk powder, milk powder, rice protein powder, milk, rice flour, yogurt, edible oil, and feed.

    Use Principles

    The Aflatoxin B1 kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin B1 antigen is precoated on the wells. The precoated antigen compete the Aflatoxin B1 antibody (antibody solution) with Aflatoxin B1 in the sample, anti-Aflatoxin B1 antibody binds to the Aflatoxin B1-HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Aflatoxin B1 concentration of the samples is derived.

    Cross Reactivity

    Aflatoxin B1 ………………………………………………….100%

    Aflatoxin B2 ………………………………………………….38%

    Aflatoxin G1 ………………………………………………….27%

    Aflatoxin G2 ………………………………………………….4%

    Aflatoxin M1 …………………………………………………20%

    Aflatoxin M2 …………………………………………………<1%

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Centrifuge

    ---- Pipette: 10mL

    ---- Balance (sensibility: 0.01g)

    ---- Rubber suction bulb

    ---- Centrifuge tube: 2mL, 10mL, 50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 × 8 wells
    6 vials of Standard1mL each
    AFB1 Enzyme Conjugate12mL
    AFB1 Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Sample Diluting Buffer40mL
    Concentrated Wash Buffer (10×)40mL
    Package Insert1
    Plate Cover Sheet2
    Testing Report1

    Note: The concentrations of the standard are 0ng/kg, 5ng/kg, 15ng/kg, 45ng/kg, 135ng/kg, 405ng/kg.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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