In Stock

Honey and Dairy Qualitative or Quantitative Quinolones ELISA Test Kit

Qualitative or quantitative analysis of quinolones in honey, milk, milk powder, etc.

Order No.: SC0039


The Quinolones (QNs) are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming. It has attracted widespread attention due to its drug resistance and potential carcinogenicity. The Maximum Residue Limit (MRL) for some quinolones has been clearly stipulated in the EU Regulations.

【Use Principles】

Quinolones ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

【Cross Reactivity】

Ciprofloxacin (CIF) 100%

Enrofloxacin (ENR) 200%

Norfloxacin (NOR) 150%

Ofloxacin (OFL) 60%

Danofloxain (DAN) 60%

Sarafloxacin (SAR) 50%

Marbolxacin (MAR) 12%

Enoxacin (ENO) 85%

Pefloxacin (PEF) 61%

Lomefloxacin (LOM) 73%

Enoxacin (ENX) 110%

Difloxacin (DIF) 50%

Amifloxacin (AMI) 75%

【Materials Required But Not Provided】

  1. Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge

—- Constant Temperature Incubator

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Rubber suction bulb

—- Graduated pipette: 10mL

—- Centrifuge tube: 2mL, 10mL, 50mL

—- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

Multi-channel pipette (30-300μL)

  1. Reagent:

—- Deionized water

—- Methanol (AR)

—- Dichloromethane (AR)

—- N-hexane (AR)

—- Sodium sulphate anhydrous (Na2SO4) (AR)

—- Sodium carbonate (Na2CO3) (AR)

—- Sodium hydrogen carbonate (NaHCO3) (AR)

—- Ethyl acetate (AR)

—- Acetonitrile (AR)

—- 18M concentrated sulfuric acid (H2SO4)

【Provided Materials and Reagent】

Component96 wells
Antigen coated plate12 × 8 wells
6 vials of standard1mL each
High Concentration Standard (1ppm)1mL
Auxiliary Extracting Agent3mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Package Insert1
Plate cover sheet2
Testing Report1

Note: The concentrations of the standards are 0μg/kg, 0.5μg/kg, 1.5μg/kg, 4.5μg/kg, 13.5μg/kg and 40.5μg/kg.


Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Aflatoxin M1 Immunoaffinity Column

Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

Aflatoxin M1 Qualitative Lateral Flow Rapid Test Kit

Aflatoxin M1 Qualitative Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of aflatoxin M1 in raw cow’s milk. This test is designed for rapid field use or daily control in laboratories.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Tetracyclines ELISA Test Kit for Animal Tissue, Seafood and Egg

Qualitative or quantitative analysis of tetracycline, oxytetracycline, aureomycin, doxycycline in animal tissue, seafood and egg, etc.Meizheng Bio-Tech Company Overview. PDF


Back to Top
Product has been added to your cart