Honey and Dairy Qualitative or Quantitative Quinolones ELISA Test Kit

【Summary】

The Quinolones (QNs) are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming. It has attracted widespread attention due to its drug resistance and potential carcinogenicity. The Maximum Residue Limit (MRL) for some quinolones has been clearly stipulated in the EU Regulations.

【Use Principles】

Quinolones ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

【Cross Reactivity】

Ciprofloxacin (CIF) 100%

Enrofloxacin (ENR) 200%

Norfloxacin (NOR) 150%

Ofloxacin (OFL) 60%

Danofloxain (DAN) 60%

Sarafloxacin (SAR) 50%

Marbolxacin (MAR) 12%

Enoxacin (ENO) 85%

Pefloxacin (PEF) 61%

Lomefloxacin (LOM) 73%

Enoxacin (ENX) 110%

Difloxacin (DIF) 50%

Amifloxacin (AMI) 75%

【Materials Required But Not Provided】

1. Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge

—- Constant Temperature Incubator

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Rubber suction bulb

—- Graduated pipette: 10mL

—- Centrifuge tube: 2mL, 10mL, 50mL

—- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

Multi-channel pipette (30-300μL)

2. Reagent:

—- Deionized water

—- Methanol (AR)

—- Dichloromethane (AR)

—- N-hexane (AR)

—- Sodium sulphate anhydrous (Na₂SO₄) (AR)

—- Sodium carbonate (Na₂CO₃) (AR)

—- Sodium hydrogen carbonate (NaHCO₃) (AR)

—- Ethyl acetate (AR)

—- Acetonitrile (AR)

—- 18M concentrated sulfuric acid (H₂SO₄)

【Provided Materials and Reagent】

Note: The concentrations of the standards are 0μg/kg, 0.5μg/kg, 1.5μg/kg, 4.5μg/kg, 13.5μg/kg and 40.5μg/kg.