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    Honey and Dairy Qualitative or Quantitative Quinolones ELISA Test Kit

    Qualitative or quantitative analysis of quinolones in honey, milk, milk powder, etc.

    Order No.: SC0039

    【Summary】

    The Quinolones (QNs) are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming. It has attracted widespread attention due to its drug resistance and potential carcinogenicity. The Maximum Residue Limit (MRL) for some quinolones has been clearly stipulated in the EU Regulations.

    【Use Principles】

    Quinolones ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The quinolones antigen is precoated on the wells. The precoated antigen compete the quinolones antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the quinolones-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

    【Cross Reactivity】

    Ciprofloxacin (CIF) 100%

    Enrofloxacin (ENR) 200%

    Norfloxacin (NOR) 150%

    Ofloxacin (OFL) 60%

    Danofloxain (DAN) 60%

    Sarafloxacin (SAR) 50%

    Marbolxacin (MAR) 12%

    Enoxacin (ENO) 85%

    Pefloxacin (PEF) 61%

    Lomefloxacin (LOM) 73%

    Enoxacin (ENX) 110%

    Difloxacin (DIF) 50%

    Amifloxacin (AMI) 75%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Vortex

    ---- Centrifuge

    ---- Constant Temperature Incubator

    ---- Termovap Sample Concentrator

    ---- Balance (sensibility: 0.01g)

    ---- Rubber suction bulb

    ---- Graduated pipette: 10mL

    ---- Centrifuge tube: 2mL, 10mL, 50mL

    ---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

    Multi-channel pipette (30-300μL)

    1. Reagent:

    ---- Deionized water

    ---- Methanol (AR)

    ---- Dichloromethane (AR)

    ---- N-hexane (AR)

    ---- Sodium sulphate anhydrous (Na2SO4) (AR)

    ---- Sodium carbonate (Na2CO3) (AR)

    ---- Sodium hydrogen carbonate (NaHCO3) (AR)

    ---- Ethyl acetate (AR)

    ---- Acetonitrile (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component96 wells
    Antigen coated plate12 × 8 wells
    6 vials of standard1mL each
    High Concentration Standard (1ppm)1mL
    Auxiliary Extracting Agent3mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Package Insert1
    Plate cover sheet2
    Testing Report1

    Note: The concentrations of the standards are 0μg/kg, 0.5μg/kg, 1.5μg/kg, 4.5μg/kg, 13.5μg/kg and 40.5μg/kg.

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