Gentamicin ELISA Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.
| Kit Contents | Quantity | Storage |
| Gentamicin-Coated Plate | 1 x 96-well plate | 2–8°C |
| Gentamicin Standards | ||
| Negative control (white cap tube) | 0.8 mL | 2–8°C |
| 0.05 ng/mL (yellow cap tube) | 0.8 mL | 2–8°C |
| 0.1 ng/mL (orange cap tube) | 0.8 mL | 2–8°C |
| 0.3 ng/mL (pink cap tube) | 0.8 mL | 2–8°C |
| 0.9 ng/mL (purple cap tube) | 0.8 mL | 2–8°C |
| 2.7 ng/mL (blue cap tube) | 0.8 mL | 2–8°C |
| Spiking Solution | ||
| 50 ng/mL (red cap tube) | 0.8 mL | 2–8°C |
| Gentamicin Antibody #1 | 12 mL | 2–8°C |
| 100X HRP-Conjugated Antibody #2 | 250 µL | 2–8°C |
| 20X Wash Solution | 28 mL | 2–8°C |
| Antibody #2 Diluent | 20 mL | 2–8°C |
| TMB Substrate | 12 mL | 2–8°C |
| 10X Sample Extraction Buffer | 25 mL | 2–8°C |
Required Materials Not Provided with the Kit
- Microtiter Plate Reader with 450 nm primary filter and optional 630 nm differentialfilter
- TissueHomogenizer
- VortexMixer
- 10, 20, 100 and 1000 µLPipettes
- Distilled or deionizedwater
- Multi-Channel Pipette: 50–300 µL(Optional)
- Reagent Resevoir(Optional)
