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Honey Nitroimidazoles ELISA Test Kit

The kit is intended for the quantitative analysis of nitroimidazoles in honey.

Brochure-Honey Test. PDF

Meizheng Bio-Tech Company Overview. PDF


Nitroimidazole antibacterial drugs are mainly used to treat and prevent infections caused by trichomoniasis, amoeba protozoa and anaerobic bacteria. However, due to the carcinogenic effects of these drugs, many countries prohibit their use in the breeding of livestock, poultry and aquatic products. Our country prohibits these drugs as growth promoters for animals.

Use Principles:

The kit is an indirect competitive enzyme-labeled immunoassay. The Nitroimidazoles antigen is precoated on the wells. The precoated antigen compete the Nitroimidazoles antibody (antibody solution) with Nitroimidazoles in the sample, anti-Nitroimidazoles antibody binds to the Nitroimidazoles-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Nitroimidazoles concentration of the samples is derived.

Order No.SC0040
Sample TypeHoney.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityMetronidazole……………………………….100%
Secnidazole, Ornidazole …………………15%
2-Nitroimidazole, Ronidazole……………<1%
Calculate ResultAnalyze the result by using the software
Sensitivity  0.5ppb
Limit of detection0.2ppb
Percent Recovery, %90±15%
Precision (CV %):Intra-lab assay: CV<10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
5 vials of standard2mL each
High concentration standard (1ppm)1mL
Enzyme Conjugate6mL
Antibody solution6mL
Substrate A6mL
Substrate B6mL
Concentrated wash buffer (10×)40mL
Concentrated sample diluting buffer50mL
Plate cover sheet2
Package insert1
Testing Report1
5 vials of standard2mL each

Note: The concentrations of the controls are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb respectively.

Materials Required But Not Provided

  1. Devices:

┅┅Microplate reader 450nm/630nm


┅┅Biochemical incubator (adjustable 4℃, 25℃)

┅┅Centrifuge (4000g and above)

┅┅Balance: Sensitivity 0.01g

┅┅Nitrogen evaporator

┅┅Centrifuge tube: 7mL, 50mL

┅┅Transfer pipette: 10mL

┅┅Micropipette: single channel 10μL-100μL, 100μL-1000μL,

Multi-channel 30μL-300μL

  1. Reagent:

—- Deionized water

—-Ethyl acetate (AR)

—-N-hexane (AR)

—-Dodecahydrate and disodium hydrogen phosphate

—-Sodium dihydrogen phosphate dihydrate

—- Concentrated sulfuric acid (18 mol/L)

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.


1-10, 11-100, 101-1000, 1001-10000


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