Background:
Nitroimidazole antibacterial drugs are mainly used to treat and prevent infections caused by trichomoniasis, amoeba protozoa and anaerobic bacteria. However, due to the carcinogenic effects of these drugs, many countries prohibit their use in the breeding of livestock, poultry and aquatic products. Our country prohibits these drugs as growth promoters for animals.
Use Principles:
The kit is an indirect competitive enzyme-labeled immunoassay. The Nitroimidazoles antigen is precoated on the wells. The precoated antigen compete the Nitroimidazoles antibody (antibody solution) with Nitroimidazoles in the sample, anti-Nitroimidazoles antibody binds to the Nitroimidazoles-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Nitroimidazoles concentration of the samples is derived.
Manufacturer | Meizheng |
Order No. | SC0040 |
Analyte | Nitroimidazoles |
Sample Type | Honey. |
Methodology | Indirect competitive enzyme-labeled immunoassay. |
Packaging | 96 Wells |
Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
Shelf life | The validity for this kit is 12 months |
Lead time | Shipped within 5-12 working days. |
Cross Reactivity | Metronidazole……………………………….100% Dimetridazole……………………………….450% Secnidazole, Ornidazole …………………15% 2-Nitroimidazole, Ronidazole……………<1% |
Calculate Result | Analyze the result by using the software |
Sensitivity | 0.5ppb |
Limit of detection | 0.2ppb |
Percent Recovery, % | 90±15% |
Precision (CV %): | Intra-lab assay: CV<10% |
Provided Materials and Reagent
Component | 96 wells |
Antigen coated plate | 12 test strips of 8 wells each |
5 vials of standard | 2mL each |
High concentration standard (1ppm) | 1mL |
Enzyme Conjugate | 6mL |
Antibody solution | 6mL |
Substrate A | 6mL |
Substrate B | 6mL |
Concentrated wash buffer (10×) | 40mL |
Concentrated sample diluting buffer | 50mL |
Plate cover sheet | 2 |
Package insert | 1 |
Testing Report | 1 |
5 vials of standard | 2mL each |
Note: The concentrations of the controls are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb respectively.
Materials Required But Not Provided
- Devices:
┅┅Microplate reader 450nm/630nm
┅┅Vortex
┅┅Biochemical incubator (adjustable 4℃, 25℃)
┅┅Centrifuge (4000g and above)
┅┅Balance: Sensitivity 0.01g
┅┅Nitrogen evaporator
┅┅Centrifuge tube: 7mL, 50mL
┅┅Transfer pipette: 10mL
┅┅Micropipette: single channel 10μL-100μL, 100μL-1000μL,
Multi-channel 30μL-300μL
- Reagent:
—- Deionized water
—-Ethyl acetate (AR)
—-N-hexane (AR)
—-Dodecahydrate and disodium hydrogen phosphate
—-Sodium dihydrogen phosphate dihydrate
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.