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    Honey Pesticides Acetamiprid ELISA Test Kit

    Order No.: SC0062

    LOD: 0.2 ppb

    Package: 96T

    [Scope of Application]

    Qualitative or quantitative analysis of acetamiprid in honey.

    [Summary]

    Acetamiprid is an organic compound. It is systemic and intended to control sucking insects (Thysanoptera, Hemiptera, mainly aphids) on crops such as leafy vegetables, citrus fruits, pome fruits, grapes, cotton, cole crops, and ornamental plants. Acetamiprid is an insecticide belonging to the chloronicotintl neonicotinoids, this compound is an insecticide that is introduced for controlling pests. It is, however, reported as a reproduction/developmental toxicant. It is moderately toxic to honeybees and most aquatic organisms. Acetamiprid is highly toxic to insects, but less to mammals.

    [Principles]

    Acetamiprid ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The acetamiprid antigen is precoated on the wells. The precoated antigen compete the acetamiprid antibody (antibody solution) with acetamiprid in the sample, anti-acetamiprid antibody binds to the acetamiprid-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the acetamiprid concentration of the samples is derived.

    [Cross Reactivity]

    Acetamiprid………………………………………………100%

     [Materials Required But Not Provided]

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Centrifuge

    ---- Constant Temperature Incubator

    ---- Balance (sensibility: 0.01g)

    ---- Rubber suction bulb

    ---- Graduated pipette: 10mL

    ---- Centrifuge tube: 2mL, 50mL

    ---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

    Multi-channel pipette (30-300μL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

     [Provided Materials and Reagent]

    Component96 wells
    Antigen coated plate12 × 8 wells
    5 vials of standard1mL each
    High Concentration Standard (1ppm)1mL
    Enzyme Conjugate12mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL*2
    Package Insert1
    Plate cover sheet2
    Testing Report1

    Note: The concentrations of the standards are 0μg/kg, 0.2μg/kg, 0.6μg/kg, 1.8μg/kg and 5.4μg/kg.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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