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Honey Qualitative or Quantitative Erythromycin ELISA Test Kit

Order No.:EC0192

Qualitative or quantitative analysis of Erythromycin in honey.

【Summary】

Erythromycin is a macrolide antibiotic, which is used to treat bacterial and mycoplasma infections. It also can be used as a feed additive to improve feed transformation efficiency and increase body weight.

Use Principles】

Erythromycin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Erythromycin antigen is precoated on the wells. The precoated antigen compete the Erythromycin antibody (antibody solution) with Erythromycin in the sample, anti-Erythromycin antibody binds to the Erythromycin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Erythromycin concentration of the samples is derived.

【Scope of Application】

Qualitative or quantitative analysis of Erythromycin in honey.

【Cross Reactivity】

Erythromycin…………………………………….…………..……100%

Tilmicosin…………………………………………………………<0.1%

Tylosin…………………………………….………………..…….<0.1%

【Materials Required but Not Provided】

  1.  Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Oscillator / Vortex

—- Constant Temperature Incubator

—- Balance (sensibility: 0.01g)

—- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

Multi-channel pipette (30-300μL)

  1.  Reagent:

—- Deionized water

—- Ethanol (AR)

—- 18M Concentrated Sulfuric Acid (H2SO4)

【Provided Materials and Reagent】

Component96 wellsStorage
Antigen coated plate12 × 8 wells2-8℃
Standards  
Negative control (white cap tube)0.8mL2-8℃
0.5μg/kg (yellow cap tube)0.8mL2-8℃
1μg/kg (orange cap tube)0.8mL2-8℃
2μg/kg (pink cap tube)0.8mL2-8℃
4μg/kg (purple cap tube)0.8mL2-8℃
8μg/kg (blue cap tube)0.8mL2-8℃
Spiking Solution  
100μg/kg (red cap tube)0.8mL2-8℃
Antibody #112mL2-8℃
HRP-Conjugated Antibody #218mL2-8℃
Concentrated Wash Buffer (20×)28mL2-8℃
TMB Substrate12mL2-8℃
PBS (10×)25mL2-8℃
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0μg/kg, 0.5μg/kg, 1μg/kg, 2μg/kg, 4μg/kg and 8μg/kg.

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