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Honey Qualitative or Quantitative Trimethoprim ELISA Test Kit

Qualitative or quantitative analysis of  trimethoprim residues in honey.

Order No.: SC0055


Trimethoprim (TMP) is a common synergist for trimethoprim. The combination use of trimethoprim and trimethoprim drugs enhances the antibacterial effect significantly. It is restricted in human consumption animals.

Use Principles 

Trimethoprim ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The trimethoprim antigen is precoated on the wells. The precoated antigen compete the trimethoprim antibody (antibody solution) with trimethoprim in the sample, anti- trimethoprim antibody binds to the trimethoprim-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the trimethoprim concentration of the samples is derived.


Trimethoprim (TMP)…………………………………………100%

Diaveridine (DVD)……………………………………………<1%

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Constant Temperature Incubator

—- Oscillator / Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Graduate pipette: 10mL

—- Rubber suction bulb

—- Termovap Sample Concentrator

—- Centrifuge tube: 2mL, 10mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30 -300µL)

  1. Reagent:

—- Deionized water

—- Ethyl acetate (AR)

—- N-Hexane (AR)

—- 18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent

Component 96 wells
Antigen coated plate12 × 8 wells
6 vials of standard2mL each
High concentration standard (1ppm)1mL
Enzyme Conjugate12mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Concentrated Auxiliary Extracting Agent50mL
Package Insert1
Testing Report1
Plate cover sheet2

Note: The concentrations of the standards are 0μg/kg, 0.5μg/kg, 1.5μg/kg, 4.5μg/kg, 13.5μg/kg and 40.5μg/kg.


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