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    Liquid Milk Antibiotic Lincomycin ELISA Test Kit

    Qualitative or quantitative analysis of Lincomycin in liquid milk. Lincomycin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay.

    Brochure-Dairy Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Lincomycin is a Lincosamides antibiotic mainly used to restrain synthesis of bacterial protein. Lincomycin residual in livestock and poultry will hurt liver and cause allergic reaction and Cardiovascular reaction.

    Use Principles:

    Lincomycin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Lincomycin antigen is precoated on the wells. The precoated antigen compete the Lincomycin antibody (antibody solution) with Lincomycin in the sample, anti- Lincomycin antibody binds to the Lincomycin -HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Lincomycin concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.SC0053
    AnalyteLincomycin
    Sample TypeLiquid milk
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityLincomycin…………………………..………100%
    Clindamycin…………………………..………<1%
    Spiramycin…………………….……..………<1%
    Erythromycin…………………………………<1%
    Tylosin………………………………..………<1%
    Streptomycin………………………..………<1%
    Kanamycin……………...…………..………<1%
    Spectinomycin…………...………..………<1%
    Calculate ResultAnalyze the result by using the software
    Sensitivity   0.5ppb
    Limit of detection2ppb
    Percent Recovery, %100±20%
    Precision (CV %):Intra-lab assay: CV<10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96T
    6 vials of standard1mL each
    High concentration standard (2ppm)1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer (5×)40mL
    Instruction1
    Testing Report1
    Plate cover sheet2
    High concentration standard (2ppm)1mL each

     Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.

    Materials Required But Not Provided

    1.  Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex

    ---- Incubator (optional if the room temperature can reach 25℃)

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipet (10μL-100μL, 100μL-1000μL)

    Multi-channel pipet (30-300μL)

    1.  Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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