Search

In Stock

Meat Antibiotic Sulfonamides ELISA Test Kit

Qualitative or quantitative analysis of sulfonamides residues in tissue, aquatic tissue, serum, etc.

Summary

Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

HPLC/GC-MS method is now widely used in the detection of sulfonamides residues, but its high cost and complex operation make it unacceptable.

Order No.: ME08EER

Sensitivity: 0.5 ppb

Package: 96T

Use Principles

Sulfonamides ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.

Cross-reactivity

Sulfadiazine 100%

Sulfadoxine 181%

Sulfamonomethoxine 150%

Phthalylsulfathiazole (PST) 90%

Sulfamethoxydiazine 85%

Sulfadimethoxypyrimidine 67%

Benzene Sulfapyridine 62%

Sulfapyridine 55%

Sulfamerazine 52%

Sulfamethizole 49%

Sulfachloropyridazine 29.6%

Sulfamethazine 13%

Materials Required But Not Provided

1.Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator

—- Vortex

—- Rotary evaporator/nitrogen evaporator

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Graduated pipette: 10mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)

  1. Reagent:

—- Methanol (AR)

—- N-hexane (AR)

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

Provided Materials and Reagent

Component96 wells
Antigen coated plate96 T
5 vials of standard1mL each
High concentration standard 1ppm1mL
SAs Enzyme Conjugate6mL
SAs Antibody Solution9mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
SAs Concentrated Sample Diluting Buffer I (10×)45mL
SAs Concentrated Sample Diluting Buffer II (2×)45mL
IFU1

Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb.

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
6 vials of standard1mL each
High concentrated standard (100ppb)1mL
AHD Enzyme Conjugate6mL
AHD Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated sample diluting buffer (2X)40mL
Concentrated derivatization reagent10X)1mL
IFU1
Testing Report1
Plate cover sheet2

Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

Bestsellers

Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Aflatoxin M1 Immunoaffinity Column

Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Streptomycin/Dihydrostreptomycin, Gentamicin and Neomycin Milk Comb Rapid Test Kit

The Streptomycin/Dihydrostreptomycin & Gentamicin & Neomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of streptomycin/dihydrostreptomycin, gentamicin and neomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

T-2 Toxin Qualitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a qualitative level for the presence of T-2 Toxin and is intended for use in grains, feed materials and feed products. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

 

Back to Top
Product has been added to your cart