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Milk and Honey Qualitative or Quantitative Streptomycin ELISA Test Kit

Qualitative or quantitative analysis of streptomycin in honey and milk.

Order No.: SC0045

Summary

Streptomycin (STR) is an antibiotic (antimycobacterial) drug, the first of a class of drugs called aminoglycosides to be discovered, and it was the first effective treatment for tuberculosis. It is derived from the actinobacterium streptomyces griseus. Streptomycin is a bactericidal antibiotic.

Adverse effects of this medicine are ototoxicity, nephrotoxicity, fetal auditory toxicity, and neuromuscular paralysis.

Use Principles

Streptomycin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The streptomycin antigen is precoated on the wells. The precoated antigen compete the streptomycin antibody (antibody solution) with streptomycin in the sample, anti-streptomycin antibody binds to the streptomycin-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the streptomycin concentration of the samples is derived.

Cross-reactivity

Streptomycin 100%

Dihydrostreptomycin 100%

Kanamycin <0.01%

Gentamycin <0.01%

Materials Required but Not Provided

  1.  Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Balance (sensibility: 0.01g)

—- Centrifuge

—- Oscillator / Vortex

—- Centrifuge tube: 2mL, 10mL, 50mL

—- Graduated pipette: 10mL

—-Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

  1. Reagent:

—- Deionized water

—- 18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent

Component 96 wells
Antigen coated plate12 × 8 wells
5 vials of standard2mL each
High Concentration Standard (1ppm)2mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL×2
Package Insert1
Plate cover sheet2
Testing Report1

Note: The concentrations of the standards are 0μg/kg, 0.2μg/kg, 0.6μg/kg, 1.8μg/kg and 5.4μg/kg.

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