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    Milk Qualitative or Quantitative Doramectin ELISA Test Kit

    Qualitative or quantitative analysis of Doramectin concentrations in raw milk.

    Order No.: SC0318

    【Summary】

    Doramectin belongs to avermectins antibiotics, which are macrolide antibiotics isolated from the fermentation product of Streptomyces and have efficient killing effects on parasitic nematodes and arthropods of animals and plants. It is 5 to 50 times more effective than common pesticides and has a long-lasting effect. Doramectin is an excellent new antibiotic for crops and livestock that is widely used in crop cultivation and animal breeding. This class of drugs includes abamectin, ivermectin, doramectin, moxidectin, eprinomectin, acetamide abamectin, etc. Which belong to highly toxic microbial pesticides.

    Use Principles

    Doramectin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Doramectin antigen is precoated on the wells. The precoated antigen compete the Doramectin antibody (antibody solution) with Doramectin in the sample, anti-Doramectin antibody binds to the Doramectin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Doramectin concentration of the samples is derived.

    【Cross Reactivity】

    Doramectin……………………………………………………..100%

    Ivermectin………………………………………………………200%

    Abamectin………………………………………………………400%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Termovap Sample Concentrator

    ---- Constant Temperature Incubator

    ---- Centrifuge

    ---- Graduated pipette: 10mL

    ---- Centrifuge tube: 7mL

    ---- Rubber suction bulb

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- Acetonitrile (AR)

    ---- Methanol (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component96 wells
    Antigen coated plate12 × 8 wells
    6 vials of standard2mL each
    Enzyme Conjugate12mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Plate cover sheet2
    Package Insert1
    Testing Report1

    Note: The concentrations of the standards are 0μg/kg, 0.4μg/kg, 1μg/kg, 3μg/kg, 9μg/kg and 27μg/kg.

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