【Use Principles】
Nicarbazine residual marker ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Nicarbazine residual marker antigen is precoated on the wells. The precoated antigen compete the Nicarbazine residual marker antibody (antibody solution) with Nicarbazine residual marker in the sample, anti- Nicarbazine residual marker antibody binds to the Nicarbazine residual marker-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Nicarbazine residual marker concentration of the samples is derived.
【Scope of Application】
Qualitative or quantitative analysis of Nicarbazine residual marker in animal tissues.
【Cross-reactivity】
Nicarbazine residual marker 100%
【Materials Required But Not Provided】
- Devices:
—- Microplate Reader (450nm/630nm filter)
—- Homogenizer
—- Vortex
—- Oscillator
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Graduated Pipette: 10mL
—- Rubber Bulb Syringe
—- Centrifuge Tube: 5mL/7mL, 50mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)
2. Reagent:
—- Deionized water
—- Acetonitrile (AR)
—- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
| Component | 96 wells |
| Antigen coated plate | 96T |
| 5 vials of standard | 1mL each |
| High concentration standard (1ppm) | 1mL |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Concentrated Sample Diluting Buffer | 30mL |
| Package Insert | 1 |
| Testing Report | 1 |
| Plate Cover Sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, and 1.35ppb.
