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    Nicarbazine Residual Marker ELISA Test Kit

    Order No.: ME45AER

    Nicarbazine is a coccidiostat used on meat chickens. It is also a wide-spectrum anti-parasitic drug approved for veterinary use, effective on Toxocara canis, Toxascaris leonina, Ancylostoma caninum, Uncinaria stenocephala, Trichuris vulpis, Dipylidium caninum, and Taenia sp. and Mesocestoides sp.

    Meizheng Bio-Tech Company Overview-V2022-1 2022.6.14

    Use Principles

    Nicarbazine residual marker ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Nicarbazine residual marker antigen is precoated on the wells. The precoated antigen compete the Nicarbazine residual marker antibody (antibody solution) with Nicarbazine residual marker in the sample, anti- Nicarbazine residual marker antibody binds to the Nicarbazine residual marker-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Nicarbazine residual marker concentration of the samples is derived.

    Scope of Application

    Qualitative or quantitative analysis of Nicarbazine residual marker in animal tissues.

    Cross-reactivity

    Nicarbazine residual marker 100%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Homogenizer

    ---- Vortex

    ---- Oscillator

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Graduated Pipette: 10mL

    ---- Rubber Bulb Syringe

    ---- Centrifuge Tube: 5mL/7mL, 50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

    2. Reagent:

    ---- Deionized water

    ---- Acetonitrile (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96T
    5 vials of standard1mL each
    High concentration standard (1ppm)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer30mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, and 1.35ppb.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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