Sulfonamides (SAs) are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.
Sulfonamides ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The SAs antigen is precoated on the wells. The precoated antigen compete the SAs antibody (antibody solution) with SAs in the sample, anti-SAs antibody binds to the SAs-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the SAs concentration of the samples is derived.
【Scope of Application】
Qualitative or quantitative analysis of Sulfonamides residues in honey.
Sulfachloropyrazine sodium 265%
【Materials Required But Not Provided】
—- Microwell Plate Reader (450nm/630nm)
—- Oscillator / Vortex
—- Balance (sensibility: 0.01g)
—- Centrifuge tube: 7mL, 10mL, 50mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
—- Deionized water
—- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||12 × 8 wells|
|5 vials of standard||1mL each|
|High Concentration Standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer||40mL|
|Plate cover sheet||2|
Note: The concentrations of the standards are 0μg/kg, 0.3μg/kg, 0.9μg/kg, 2.7μg/kg, 8.1μg/kg.