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    Qualitative or Quantitative Sulfonamides ELISA Test Kit for Honey

    Order No.: EC0066

    Qualitative or quantitative analysis of Sulfonamides residues in honey.

    Summary 

    Sulfonamides (SAs) are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

    Use Principles 

    Sulfonamides ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The SAs antigen is precoated on the wells. The precoated antigen compete the SAs antibody (antibody solution) with SAs in the sample, anti-SAs antibody binds to the SAs-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the SAs concentration of the samples is derived.

    Scope of Application

    Qualitative or quantitative analysis of Sulfonamides residues in honey.

    Cross-reactivity

    Sulfadiazine 100%

    Sulfamethazine 13%

    Sulfamonomethoxine 171%

    Sulfaquinoxaline 15%

    Sulfachloropyridazine 21%

    Sulfachloropyrazine sodium 265%

    Sulfadimoxine 132%

    Sulfamerazine 42%

    Sulfadoxine 133%

    Sulfadimethoxine 107%

    Phthalylsulfathiazole 11%

    Sulfamoppyridine 8%

    Sulfapyridine 7%

    Sulfamethizole 65%

    Sulfamidinum 6%

    Sulfamethoxazole 3%

    Materials Required But Not Provided

    1.  Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Balance (sensibility: 0.01g)

    ---- Centrifuge tube: 7mL, 10mL, 50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    Provided Materials and Reagent

    Component 96 wells
    Antigen coated plate12 × 8 wells
    5 vials of standard1mL each
    High Concentration Standard (1ppm)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Package Insert1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0μg/kg, 0.3μg/kg, 0.9μg/kg, 2.7μg/kg, 8.1μg/kg.

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