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Raw Milk and Dairy Aflatoxin M1 ELISA Test Kit

The Aflatoxin M1 ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Aflatoxin M1 concentrations in liquid milk, fermented milk, milk power, cheese, etc.


Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cotton seed and human blood.

Aflatoxin B1 is a potent human carcinogen and may contribute to human liver cancer.

Aflatoxin M1 is one of Aflatoxin with similar structure  compounds. On damp-heat areas, the occurrence of Aflatoxin is highest in food and feed. Aflatoxin M1 with quite stable physical and chemistry properties, is not destroyed by pasteurization. When the mammal animal ingests food or feed containing Aflatoxin B1, it can be converted to the Aflatoxin M1 by hydroxylation. The main harm of Aflatoxin M1 is carcinogenicity and mutagenicity, destroying human and animal liver tissue, resulting in hepatocarcinoma and even death.

Use Principles:

The Aflatoxin M1 kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin M1 antigen is precoated on the wells. The aflatoxin M1 in the sample compete the aflatoxin M1 antibody (antibody solution) with aflatoxin M1 antigen on the well, while the M1 antibody combines with the enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Aflatoxin M1 concentration of the samples is derived.

Order No.SC0033
AnalyteAflatoxin M1
Sample TypeRaw milk, Milk products
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityAFM1:100%


Calculate ResultAnalyze the result by using the software
Sensitivity   0.015 ppb
Limit of detection 0.015 ppb
Percent Recovery, %100±20%
Precision (CV %):Intra-lab assay:  CV%≤10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 X 8 wells each
5 vials of Standard1mL each
AFM1 Enzyme Conjugate6mL
AFM1 Antibody solution6mL
Substrate A6mL
Substrate B6mL
Concentrated wash buffer (10X)40mL
Concentrated sample diluting buffer I (4X)50mLX2
Concentrated sample diluting buffer II (8X)15mL
Plate cover sheet2
Package insert1

Note: The concentrations of the standards are 0μg/kg, 0.015μg/kg, 0.045μg/kg, 0.15μg/kg and 0.5μg/kg respectively.

Materials Required But Not Provided

1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge /funnel

—- Balance (sensibility: 0.01g)

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL). Multi-channel pipet (30-300µL)

—- Triangular flask

—- nitrogen evaporator

—- Graduated pipette (10mL)

—- Centrifuge tube

2. Reagent:

—- Deionized water

—- Methanol (AR)

—- Concentrated sulfuric acid (18 mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.


1-10, 11-100, 101-1000, 1001-10000


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