Sulfonamides (SAs) ELISA Kit for Animal Tissue, Eggs and Swine Urine

Summary

Sulfonamides (SAs) are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

HPLC/GC-MS method is now widely used in the detection of sulfonamides residues, but its high cost and complex operation make it unacceptable.

Use Principles

Sulfonamides (SAs) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.

Cross-reactivity

Sulfamerazine………………………………………………100%

Sulfadiazine…………………………………………………155%

Sulfamonomethoxine…………………………………408%

Sulfaquinoxaline………………………………………23%

Sulfachloropyridazine……………………………….51%

Sulfaclozine…………………………………………….40%

Sulfadimethoxine…………………………………..242%

Sulfameter…………………………………………….253%

Sulfamethazine……………………………………….38%

Sulfadoxine………………………………………………285%

Phthalylsulfathiazole (PST)…………………………42%

Sulfamethoxypyridazine……………….……………40%

Sulfisomidine………………………………………..98%

Sulfamethizole……………………………………….125%

Sulfathiazole…………………………………………….41%

Sulfabenzamide……………………………………….39%

Materials Required But Not Provided

1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator

—- Vortex

—- Rotary evaporator/nitrogen evaporator

—- Centrifuge

—- Centrifuge tube: 2mL, 10mL/ 7mL, 50mL

—- Balance (sensibility: 0.01g)

—- Graduated pipette: 10mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)

2. Reagent:

—- Methanol (AR)

—- N-hexane (AR)

—- Deionized water

—–Ethyl acetate (AR)

—–Neutral alumina (100-200mesh)

—–Acetonitrile (AR)

—- Concentrated sulfuric acid (18 mol/L)

Provided Materials and Reagent

Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.