The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2, M1, M2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.
1. Principle:
The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the toxin in the sample is extracted, filtered, and diluted, and then passed slowly through the immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. The toxins are then eluted with eluent and injected into an analytical instrument for detection.
2. Components of the kit:
Each kit contains 2-in-1 Aflatoxin (B1,B2,G1,G2,M1,M2)/Ochratoxin A immunoaffinity columns and 1 instruction manual.
3. Necessary items not provided in the box:
- Equipment
- LC-MS(LC-MS/MS)
- Centrifuge capable of at least 3,000-4,000 xg
- Nitrogen gas evaporatorapparatus
- Nitrogen gas tank and pressureregulator
- Air-pressure controllerbracket
- Airpump
- Balance with 0.01greadability
- High-speed homogenizer (maximum speed> 10,000 r/ min) or shaker
- Grinder
- Sievingscreen:2-mm
- Graduated cylinder: 100 mL/10mL
- Funnel: 50mL
- Syringe: 10 mL/20mL
- Pipette: 1 mL and pipettetips
- Homogenization flask (or 250-mL conical flask withpestle)
- Vials andtubes
- Rapid qualitative filterpaper
- Microfiber filter paper (e.g., Whatman934-AH)
- Column holder and syringe connector plug (for use with immunoaffinity columns)
Reagents
- Methanol (CH3OH): Chromatographicgrade
- Acetic acid (CH3COOH): ChromatographyGrade
- Sodium chloride (NaCl): AnalyticalGrade
- TWEEN-20 (C58H114O26): AnalyticalGrade
- Sodium chloride (NaCl): Analytical Grade
- TWEEN-20 (C58H114O26): Analytical Grade
- Sodium hydroxide (NaOH): AnalyticalGrade
- Hydrochloric acid (HCl): AnalyticalGrade
- Distilled/deionized water
Note:
- Allow the immunoaffinity column to return to room temperature (22 to 25°C) before
- The affinity column should be stored at 2 to 8°C, do not
- Dot use any expired immunoaffinity
- The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted
- Columncapacity:
Toxin name | Column Capacity: ng |
OTA | 100 |
AFT B1 |
300 |
AFT B2 | |
AFT G1 | |
AFT G2 | |
AFT M1 | 100 |
AFT M2 | 100 |