Thiamphenicol is a widely used antibiotic, illegal additions exist in the livestock industry. Excessive use of this drug will lead to high concentrations of drug residues in livestock and poultry products, which will directly affect the health of consumers. The detection method is sensitive, fast, simple, and specific, only need 45 minutes for operating and a small amount of sampling.
The Thiamphenicol ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of thiamphenicol in samples of animal tissues, eggs etc.
The Thiamphenicol ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with thiamphenicol in the sample, anti-thiamphenicol antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the thiamphenicol concentration of the samples is derived.
【Materials Required But Not Provided】
—- Microplate Reader (450nm/630nm filter)
—- Constant temperature incubator
—- Termovap Sample Concentrator
—- Balance (sensibility: 0.01g)
—- Graduated pipette: 10mL
—- Centrifuge tube (5mL or 7mL, 50mL)
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
—- Deionized water
—- Acetonitrile (AR)
—- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||96 T|
|6 vials of Standard||1mL each|
|High concentration standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Sample Diluting Buffer ×2 Bottles||45mL/Bottle|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 1ppb, 3ppb, 9ppb, 27ppb, 81ppb respectively.