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Animal Tissue Antibiotic Chloramphenicol ELISA Test Kit

Qualitative or quantitative analysis of Chloramphenicol in animal tissue (chicken, pork, fish, shrimp, duck, bullfrog).

Summary

Chloramphenicol (CAP) is a widely used antibiotic, but has serious side effects, which can cause aplastic anemia, agranulocytosis and other diseases.

The detection method is sensitive, fast, simple and specific, only need 45min for operating and a small amount of sampling.

Order NO.: ME01AER

LOD: 0.02 ppb

Samples: Meat, Seafood, Liver, Egg, Swine urine, Feeds

Use Principles

Chloramphenicol ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Chloramphenicol in the sample, anti-Chloramphenicol antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Chloramphenicol concentration of the samples is derived.

Cross-reactivity

Chloramphenicol…………………………………………100%

Chloramphenicol Base…………………………………0.5%

Methyl Chloramphenicol………………………………<0.05%

Florfenicol……………………………………………………<0.01%

Florfenicolamide…………………………………………<0.1%

Materials Required But Not Provided

Devices:

—- Microplate Reader (450nm/630nm filter)

—- Oscillator

—- Vortex

—- Centrifuge

—- Homogenizer

—- Constant Temperature Incubator

—- Water Bath

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Centrifuge Tube: 10mL or 7mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

Reagent:

—- Deionized Water

—- Ethyl Acetate (AR)

—- N-hexane (AR)

—- Methanol (AR)

—- Concentrated hydrochloric acid (HCl) (AR)

—–Sodium hydroxide (NaOH) (AR)

—- Dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O) (AR)

—- 18M concentrated sulfuric acid (H2SO4)

[Provided Materials and Reagent]

Component96 wells
Antigen Coated Plate96T
6 vials of Standard1mL each
High Concentration Standard (100ppb)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer (2×)40mL
Concentrated Derivatization Reagent (10×)1mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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