Gentamicin (GEN) belongs to aminoglycoside antibiotics and mainly acts on Gram-negative bacteria. It is widely used in veterinary clinics and animal feed additives due to its broad antibacterial spectrum, good efficacy and low price. In the process, it is easy to produce toxic and side effects, especially the use of large doses is not standardized, which can easily lead to excessive GEN residues in animal food, which poses a hazard to human health, and seriously damages brain nerves, hearing and kidneys. Existing methods for detecting gentamicin, such as chromatography or chromatography-mass spectrometry, are complicated in sample preparation, expensive in equipment, complicated in operation, and time-consuming.
The Gentamicin ELISA kit can be used to quickly and accurately analyze gentamicin residues in samples. This kit is a drug residue detection product developed by ELISA technology. Compared with instrument analysis technology, it has the advantages of rapidity, simplicity and high sensitivity, and can minimize the work intensity.
Gentamicin ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The gentamicin antigen is precoated on the wells. The precoated antigen compete the gentamicin antibody (Antibody Solution) with Gentamicin in the sample, gentamicin antibody binds to the gentamicin Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the gentamicin contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of gentamicin in the sample can be calculated.
【Materials Required But Not Provided】
—- Microplate Reader (450nm/630nm filter)
—- Centrifuge tube: 5mL/7mL, 50mL
—- Graduated Pipette: 10mL
—- Rubber Bulb Syringe
—- Balance (sensibility: 0.01g)
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
—- Deionized or distilled water
—- Sodium Hydroxide (NaOH) (AR)
—- Trichloroacetic Acid (C2HCl3O2) (AR)
—- 18M Concentrated Sulfuric Acid (H2SO4)
【Provided Materials and Reagent】
|Antigen Coated Plate||96T|
|5 vials of Standard||1mL each|
|High Concentration Standard (100ppb)||1mL|
|Concentrated Sample Diluting Buffer||40mL|
|Concentrated Wash Buffer (10×)||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb.