Dimetridazole is an anti-fungal and anti-protozoal drug traditionally used in veterinary for the prevention and treatment of histomoniasis in turkeys, genital trichomoniasis in cattle and hemorrhagic enteritis in pigs. Results from the in vitro and in vivo tests suggested, that dimetridazole was not genotoxic compound, but its use is legally limited, which belongs to a kind of prohibited substances in the EU regulation.
Dimetridazole ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Dimetridazole antigen is precoated on the wells. The precoated antigen compete the Dimetridazole antibody (Antibody Solution) with Dimetridazole in the sample, Dimetridazole antibody binds to the Dimetridazole Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Dimetridazole contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Dimetridazole in the sample can be calculated.
【Scope of Application】
Qualitative or quantitative analysis of Dimetridazole in samples of animal tissues, eggs etc.
【Materials Required But Not Provided】
---- Microplate Reader (450nm/630nm filter)
---- Termovap Sample Concentrator
---- Balance (sensibility: 0.01g)
---- Graduated Pipette: 10mL
---- Rubber Bulb Syringe
---- Centrifuge Tube: 5mL/7mL,50mL
---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)
---- Deionized water
---- Sodium hydroxide (NaOH) (AR)
---- Ethyl acetate (AR)
---- N-hexane (AR))
---- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen Coated Plate||96T|
|6 vials of Standard||2mL each|
|High Concentrated Standard (100ppb)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer||50mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.