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    Dimetridazole ELISA Test Kit for Animal Tissues and Eggs

    Summary

    Dimetridazole is an anti-fungal and anti-protozoal drug  traditionally used in veterinary for the prevention and treatment of histomoniasis in turkeys, genital trichomoniasis in cattle and hemorrhagic enteritis in pigs. Results from the in vitro and in vivo tests suggested, that dimetridazole was not genotoxic compound, but its use is legally limited, which belongs to a kind of prohibited substances in the EU regulation.

    Use Principles

    Dimetridazole ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Dimetridazole antigen is precoated on the wells. The precoated antigen compete the Dimetridazole antibody (Antibody Solution) with Dimetridazole in the sample, Dimetridazole antibody binds to the Dimetridazole Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Dimetridazole contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Dimetridazole in the sample can be calculated.

    Scope of Application

    Qualitative or quantitative analysis of Dimetridazole in samples of animal tissues, eggs etc.

    Cross-reactivity

    Dimetridazole 100%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Homogenizer

    ---- Vortex

    ---- Centrifuge

    ---- Termovap Sample Concentrator

    ---- Balance (sensibility: 0.01g)

    ---- Graduated Pipette: 10mL

    ---- Rubber Bulb Syringe

    ---- Centrifuge Tube: 5mL/7mL,50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

    2. Reagent:

    ---- Deionized water

    ---- Sodium hydroxide (NaOH) (AR)

    ---- Ethyl acetate (AR)

    ---- N-hexane (AR))

    ---- 18M concentrated sulfuric acid (H2SO4)

    Provided Materials and Reagent

    Antigen Coated Plate96T
    6 vials of Standard2mL each
    High Concentrated Standard (100ppb)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer50mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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