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Feed Mycotoxin Zearalenone (ZEN) ELISA Test Kit

The Zearalenone ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Zearalenone in feed and feed material.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Zearalenone (ZEN) is a potent fungal toxin that affects crops grown or stored in cool and moist conditions. Zearalenone is known to cause estrogenic effects such as precocious development in swine. The toxin is formed by the Fusarium species and mainly affects corn, wheat, sorghum, barley, and rye. Such Fusarium species are known to co-produce other toxins such as deoxynivalenol.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM1796
AnalyteZearalenone
Sample TypeFeed and feed material.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityZearalenone……………100%
Zearalanone……………13%
Zeranol…………………<1%
Result InterpretationAnalyze the result by using the software
Limit of quantitation50 ppb
Limit of detection20ppb
Percent Recovery  100±20%
Intra-lab assayCV%≤10%

Materials Required But Not Provided:

Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator/ Vortex

—- Thermostat incubator

—- Centrifuge (or quantitative filter)

—- Balance (sensibility: 0.01g)

—- Pipette: 50mL

—- Triangular flask (with stopper): 100mL

—- Centrifuge tube: 7mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)

Reagent:

—- Methanol (AR)

—- Deionized water

—-18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent:

ComponentSpecs
Antigen coated plate12 × 8 wells each
5 vials of Standard*1mL each
ZEN Enzyme Conjugate12mL
ZEN Antibody Solution12mL
Substrate10mL×2
Concentrated Wash Buffer (10 ×)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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