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    Feed Mycotoxin Zearalenone (ZEN) ELISA Test Kit

    The Zearalenone ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Zearalenone in feed and feed material.

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Zearalenone (ZEN) is a potent fungal toxin that affects crops grown or stored in cool and moist conditions. Zearalenone is known to cause estrogenic effects such as precocious development in swine. The toxin is formed by the Fusarium species and mainly affects corn, wheat, sorghum, barley, and rye. Such Fusarium species are known to co-produce other toxins such as deoxynivalenol.

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM1796
    AnalyteZearalenone
    Sample TypeFeed and feed material.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityZearalenone……………100%
    Zearalanone……………13%
    Zeranol…………………<1%
    Result InterpretationAnalyze the result by using the software
    Limit of quantitation50 ppb
    Limit of detection20ppb
    Percent Recovery  100±20%
    Intra-lab assayCV%≤10%

    Materials Required But Not Provided:

    Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator/ Vortex

    ---- Thermostat incubator

    ---- Centrifuge (or quantitative filter)

    ---- Balance (sensibility: 0.01g)

    ---- Pipette: 50mL

    ---- Triangular flask (with stopper): 100mL

    ---- Centrifuge tube: 7mL

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30µL-300µL)

    Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    ----18M concentrated sulfuric acid (H2SO4)

    Provided Materials and Reagent:

    ComponentSpecs
    Antigen coated plate12 × 8 wells each
    5 vials of Standard*1mL each
    ZEN Enzyme Conjugate12mL
    ZEN Antibody Solution12mL
    Substrate10mL×2
    Concentrated Wash Buffer (10 ×)40mL
    Package Insert1
    Plate Cover Sheet2
    Testing Report1

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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