Background:
Zearalenone (ZEN) is a potent fungal toxin that affects crops grown or stored in cool and moist conditions. Zearalenone is known to cause estrogenic effects such as precocious development in swine. The toxin is formed by the Fusarium species and mainly affects corn, wheat, sorghum, barley, and rye. Such Fusarium species are known to co-produce other toxins such as deoxynivalenol.
| Manufacturer | Meizheng |
| Trademark | ToxinFast® |
| Order No. | HEM1796 |
| Analyte | Zearalenone |
| Sample Type | Feed and feed material. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Zearalenone……………100% Zearalanone……………13% Zeranol…………………<1% |
| Result Interpretation | Analyze the result by using the software |
| Limit of quantitation | 50 ppb |
| Limit of detection | 20ppb |
| Percent Recovery | 100±20% |
| Intra-lab assay | CV%≤10% |
Materials Required But Not Provided:
Devices:
—- Microwell plate Reader (450nm/630nm)
—- Oscillator/ Vortex
—- Thermostat incubator
—- Centrifuge (or quantitative filter)
—- Balance (sensibility: 0.01g)
—- Pipette: 50mL
—- Triangular flask (with stopper): 100mL
—- Centrifuge tube: 7mL
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL-300µL)
Reagent:
—- Methanol (AR)
—- Deionized water
—-18M concentrated sulfuric acid (H2SO4)
Provided Materials and Reagent:
| Component | Specs |
| Antigen coated plate | 12 × 8 wells each |
| 5 vials of Standard* | 1mL each |
| ZEN Enzyme Conjugate | 12mL |
| ZEN Antibody Solution | 12mL |
| Substrate | 10mL×2 |
| Concentrated Wash Buffer (10 ×) | 40mL |
| Package Insert | 1 |
| Plate Cover Sheet | 2 |
| Testing Report | 1 |
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
