Diclazuril ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Diclazuril antigen is precoated on the wells. The precoated antigen compete the Diclazuril antibody (antibody solution) with Diclazuril in the sample, anti-Diclazuril antibody binds to the Diclazuril-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Diclazuril concentration of the samples is derived.
【Scope of Application】
Qualitative or quantitative analysis of Diclazuril in poultry and feed.
【Materials Required But Not Provided】
—- Microplate Reader (450nm/630nm filter)
—- Constant Temperature Incubator
—- Balance (sensibility: 0.01g)
—- Centrifuge Tube: 7mL/10mL, 50mL
—- Graduated Pipette: 10mL
—- Rubber Bulb Syringe
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)
—- Deionized water
—- Acetonitrile (AR)
—- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||96T|
|5 vials of standard||1mL each|
|High Concentration Standard (1ppm)||1mL each|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 1ppb, 3ppb, 9ppb, and 27ppb.